Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage

Haroon, Mohamed F., Hu, Shihu, Shi, Ying, Imelfort, Michael, Keller, Jurg, Hugenholtz, Philip, Yuan, Zhiguo and Tyson, Gene W. (2013) Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage. Nature, 500 7464: 567-570. doi:10.1038/nature12375

Author Haroon, Mohamed F.
Hu, Shihu
Shi, Ying
Imelfort, Michael
Keller, Jurg
Hugenholtz, Philip
Yuan, Zhiguo
Tyson, Gene W.
Title Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage
Journal name Nature   Check publisher's open access policy
ISSN 0028-0836
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1038/nature12375
Open Access Status
Volume 500
Issue 7464
Start page 567
End page 570
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing
Language eng
Subject 1000 General
Abstract Anaerobic oxidation of methane (AOM) is critical for controlling the flux of methane from anoxic environments. AOM coupled to iron, manganese and sulphate reduction have been demonstrated in consortia containing anaerobic methanotrophic (ANME) archaea. More recently it has been shown that the bacterium Candidatus 'Methylomirabilis oxyfera' can couple AOM to nitrite reduction through an intra-aerobic methane oxidation pathway. Bioreactors capable of AOM coupled to denitrification have resulted in the enrichment of 'M. oxyfera' and a novel ANME lineage, ANME-2d. However, as 'M. oxyfera' can independently couple AOM to denitrification, the role of ANME-2d in the process is unresolved. Here, a bioreactor fed with nitrate, ammonium and methane was dominated by a single ANME-2d population performing nitrate-driven AOM. Metagenomic, single-cell genomic and metatranscriptomic analyses combined with bioreactor performance and 13C- and 15N-labelling experiments show that ANME-2d is capable of independent AOM through reverse methanogenesis using nitrate as the terminal electron acceptor. Comparative analyses reveal that the genes for nitrate reduction were transferred laterally from a bacterial donor, suggesting selection for this novel process within ANME-2d. Nitrite produced by ANME-2d is reduced to dinitrogen gas through a syntrophic relationship with an anaerobic ammonium-oxidizing bacterium, effectively outcompeting 'M. oxyfera' in the system. We propose the name Candidatus 'Methanoperedens nitroreducens' for the ANME-2d population and the family Candidatus 'Methanoperedenaceae' for the ANME-2d lineage. We predict that 'M. nitroreducens' and other members of the 'Methanoperedenaceae' have an important role in linking the global carbon and nitrogen cycles in anoxic environments.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP0666762
Institutional Status UQ
Additional Notes Published online: 28 July 2013.

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Created: Wed, 31 Jul 2013, 20:29:04 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences