Detection of novel polyomaviruses, TSPyV, HPyV6, HPyV7, HPyV9 and MWPyV in feces, urine, blood, respiratory swabs and cerebrospinal fluid

Rockett, Rebecca J., Sloots, Theo P., Bowes, Sharleen, O'Neill, Nicholas, Ye, Suifang, Robson, Jenny, Whiley, David M., Lambert, Stephen B., Wang, David, Nissen, Michael D. and Bialasiewicz, Seweryn (2013) Detection of novel polyomaviruses, TSPyV, HPyV6, HPyV7, HPyV9 and MWPyV in feces, urine, blood, respiratory swabs and cerebrospinal fluid. PLoS ONE, 8 5: e62764.1-e62764.9. doi:10.1371/journal.pone.0062764


Author Rockett, Rebecca J.
Sloots, Theo P.
Bowes, Sharleen
O'Neill, Nicholas
Ye, Suifang
Robson, Jenny
Whiley, David M.
Lambert, Stephen B.
Wang, David
Nissen, Michael D.
Bialasiewicz, Seweryn
Title Detection of novel polyomaviruses, TSPyV, HPyV6, HPyV7, HPyV9 and MWPyV in feces, urine, blood, respiratory swabs and cerebrospinal fluid
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-05-08
Year available 2013
Sub-type Article (original research)
DOI 10.1371/journal.pone.0062764
Open Access Status DOI
Volume 8
Issue 5
Start page e62764.1
End page e62764.9
Total pages 9
Place of publication San Francisco, United States
Publisher Public Library of Science
Collection year 2014
Language eng
Abstract Eight novel human polyomaviruses have been discovered since 2007. Prevalence rates and tissue tropism for the most recent members HPyV 6, 7, 9, TSPyV and MWPyV are largely unknown. We used real-time PCR to determine the presence of HPyV 6, 7, 9, TSPyV and MWPyV in feces (n = 263), urine (n = 189), blood (n = 161), respiratory swabs (n = 1385) and cerebrospinal fluid (n = 171) from both healthy control children and children and adults undergoing diagnostic testing. Whole genome sequencing was able to be performed on 9 MWPyV positive specimens. Novel polyomaviruses were only detected in respiratory swabs and feces, with no detections of HPyV 9 in any sample type. MWPyV was found to be the most prevalent novel polyomavirus, being detected in 18 (1.5%) respiratory specimens from symptomatic patients, 16 (9.8%) respiratory sample from healthy control children, 11 (5.9%) fecal specimens from patient suffering gastrointestinal illness, and in 13 (15.3%) of feces from healthy control children. MWPyV was found only in respiratory and fecal specimens from children, the oldest being 9 years old. HPyV 6, 7, 9 and TSPyV were also detected in respiratory specimens and fecal specimens at low prevalence (<1.3%). The majority of these detections were found in immunocompromised patients. Our findings suggest that MWPyV can result in a subclinical infection, persistent or intermittent shedding, particularly in young children. The other novel polyomaviruses were also found in respiratory and fecal specimens, but at lower prevalence and most commonly in immunocompromised individuals.
Keyword Merkel cell polyomavirus
Transcriptase-Pcr assay
Real-time Pcr
Bk virus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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