Endosomal sorting of GLUT4 and Gap1 is conserved between yeast and insulin-sensitive cells

Shewan, Annette M., McCann, Rebecca K., Lamb, Christopher A., Stirrat, Laura, Kioumourtzoglou, Dimitrios, Adamson, Iain S., Verma, Suzie, James, David E. and Bryant, Nia J. (2013) Endosomal sorting of GLUT4 and Gap1 is conserved between yeast and insulin-sensitive cells. Journal of Cell Science, 126 7: 1576-1582. doi:10.1242/jcs.114371

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Author Shewan, Annette M.
McCann, Rebecca K.
Lamb, Christopher A.
Stirrat, Laura
Kioumourtzoglou, Dimitrios
Adamson, Iain S.
Verma, Suzie
James, David E.
Bryant, Nia J.
Title Endosomal sorting of GLUT4 and Gap1 is conserved between yeast and insulin-sensitive cells
Journal name Journal of Cell Science   Check publisher's open access policy
ISSN 0021-9533
Publication date 2013-04-01
Year available 2013
Sub-type Article (original research)
DOI 10.1242/jcs.114371
Open Access Status File (Publisher version)
Volume 126
Issue 7
Start page 1576
End page 1582
Total pages 7
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Language eng
Formatted abstract
The insulin-regulated trafficking of the facilitative glucose transporter GLUT4 in human fat and muscle cells and the nitrogen-regulated trafficking of the general amino acid permease Gap1 in the yeast Saccharomyces cerevisiae share several common features: Both Gap1 and GLUT4 are nutrient transporters that are mobilised to the cell surface from an intracellular store in response to an environmental cue; both are polytopic membrane proteins harbouring amino acid targeting motifs in their C-terminal tails that are required for their regulated trafficking; ubiquitylation of both Gap1 and GLUT4 plays an important role in their regulated trafficking, as do the ubiquitinbinding GGA (Golgi-localised, γ-ear-containing, ARF-binding) adaptor proteins. Here, we find that when expressed heterologously in yeast, human GLUT4 is subject to nitrogen-regulated trafficking in an ubiquitin-dependent manner similar to Gap1. In addition, by expressing a GLUT4/Gap1 chimeric protein in adipocytes we show that the carboxy-tail of Gap1 directs intracellular sequestration and insulin-regulated trafficking in adipocytes. These findings demonstrate that the trafficking signals and their cognate molecular regulatory machinery that mediate regulated exocytosis of membrane proteins are conserved across evolution
Keyword Endosomes
Protein sorting
Regulated traffic
Amino Acid Permease
Glucose Transporter Glut4
Late Secretory Pathway
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 081933/Z/07/Z
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
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