Acquisition of the Sda1-encoding bacteriophage does not enhance virulence of the serotype M1 Streptococcus pyogenes strain SF370

Venturini, Carola, Ong, Cheryl-Iynn Y., Gillen, Christine M., Ben-Zakour, Nouri L., Maamary, Peter G., Nizet, Victor, Beatson, Scott A. and Walker, Mark J. (2013) Acquisition of the Sda1-encoding bacteriophage does not enhance virulence of the serotype M1 Streptococcus pyogenes strain SF370. Infection and Immunity, 81 6: 2062-2069. doi:10.1128/IAI.00192-13

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ303914_OA.pdf Full text (open access) application/pdf 2.24MB 0

Author Venturini, Carola
Ong, Cheryl-Iynn Y.
Gillen, Christine M.
Ben-Zakour, Nouri L.
Maamary, Peter G.
Nizet, Victor
Beatson, Scott A.
Walker, Mark J.
Title Acquisition of the Sda1-encoding bacteriophage does not enhance virulence of the serotype M1 Streptococcus pyogenes strain SF370
Formatted title
Acquisition of the Sda1-encoding bacteriophage does not enhance virulence of the serotype M1 Streptococcus pyogenes strain SF370
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2013-06-01
Sub-type Article (original research)
DOI 10.1128/IAI.00192-13
Open Access Status File (Publisher version)
Volume 81
Issue 6
Start page 2062
End page 2069
Total pages 8
Editor A. Camilli
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The resurgence of invasive disease caused by Streptococcus pyogenes (group A Streptococcus [GAS]) in the past 30 years has paralleled the emergence and global dissemination of the highly virulent M1T1 clone. The GAS M1T1 clone has diverged from the ancestral M1 serotype by horizontal acquisition of two unique bacteriophages, encoding the potent DNase Sda1/SdaD2 and the superantigen SpeA, respectively. The phage-encoded DNase promotes escape from neutrophil extracellular traps and is linked to enhanced virulence of the M1T1 clone. In this study, we successfully used in vitro lysogenic conversion to transfer the Sda1-encoding phage from the M1T1 clonal strain 5448 to the nonclonal M1 isolate SF370 and determined the impact of this horizontal gene transfer event on virulence. Although Sda1 was expressed in SF370 lysogens, no capacity of the phage-converted strain to survive human neutrophil killing, switch to a hyperinvasive covRS mutant form, or cause invasive lethal infection in a humanized plasminogen mouse model was observed. This work suggests that the hypervirulence of the M1T1 clone is due to the unique synergic effect of the M1T1 clone bacteriophage-specific virulence factor Sda1 acting in concert with the M1T1 clone-specific genetic scaffold.
Keyword Group-A Streptococcus
Cysteine protease
Invasive disease
Gene-transfer
In-vivo
Expression
Infection
Pathogenesis
Emergence
Clone
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 28 Jun 2013, 20:02:43 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences