Advanced Pulse EPR Methods for the Characterization of Metalloproteins

Harmer, Jeffrey, Mitrikas, George and Schweiger, Arthur (2009). Advanced Pulse EPR Methods for the Characterization of Metalloproteins. In Lawrence Berliner and Graeme Hanson (Ed.), High Resolution EPR: Applications to Metalloenzymes and Metals in Medicine (pp. 13-61) New York , NY, U.S.A.: Springer New York. doi:10.1007/978-0-387-84856-3_2


Author Harmer, Jeffrey
Mitrikas, George
Schweiger, Arthur
Title of chapter Advanced Pulse EPR Methods for the Characterization of Metalloproteins
Title of book High Resolution EPR: Applications to Metalloenzymes and Metals in Medicine
Place of Publication New York , NY, U.S.A.
Publisher Springer New York
Publication Year 2009
Sub-type Research book chapter (original research)
DOI 10.1007/978-0-387-84856-3_2
Open Access Status Not yet assessed
Series Biological Magnetic Resonance
ISBN 9780387848556
9780387848563
0387848568
038784855X
ISSN 0192-6020
Editor Lawrence Berliner
Graeme Hanson
Volume number 28
Chapter number 2
Start page 13
End page 61
Total pages 49
Total chapters 14
Language eng
Abstract/Summary Electron Spin Echo Envelope Modulation (ESEEM) and pulse Electron Nuclear Double Resonance (ENDOR) experiments are considered to be two cornerstones of pulse EPR spectroscopy. These techniques are typically used to obtain the static spin Hamiltonian parameters of powders, frozen solutions, and single crystals. The development of new methods based on these two effects is mainly driven by the need for higher resolution, and therefore, a more accurate estimation of the magnetic parameters. In this chapter, we describe the inner workings of ESEEM and pulse ENDOR experiments as well as the latest developments aimed at resolution and sensitivity enhancement. The advantages and limitations of these techniques are demonstrated through examples found in the literature, with an emphasis on systems of biological relevance.
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Book Chapter
Collection: Centre for Advanced Imaging Publications
 
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Created: Thu, 27 Jun 2013, 19:31:26 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging