Delivery of a multivalent scrambled antigen vaccine induces broad spectrum immunity and protection against tuberculosis

West, Nicholas P., Thomson, Scott A., Triccas, James A., Medveczky, C. Jill, Ramshaw, Ian A. and Britton, Warwick J. (2011) Delivery of a multivalent scrambled antigen vaccine induces broad spectrum immunity and protection against tuberculosis. Vaccine, 29 44: 7759-7765. doi:10.1016/j.vaccine.2011.07.109


Author West, Nicholas P.
Thomson, Scott A.
Triccas, James A.
Medveczky, C. Jill
Ramshaw, Ian A.
Britton, Warwick J.
Title Delivery of a multivalent scrambled antigen vaccine induces broad spectrum immunity and protection against tuberculosis
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
1873-2518
Publication date 2011-10-01
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2011.07.109
Open Access Status Not yet assessed
Volume 29
Issue 44
Start page 7759
End page 7765
Total pages 7
Place of publication London, United Kingdom
Publisher Elsevier Ltd
Language eng
Formatted abstract
 The development of effective anti-Tuberculosis (TB) vaccines is an important step towards improved control of TB in high burden countries. Subunit vaccines are advantageous in terms of safety, particularly in the context of high rates of HIV co-infection, but they must contain sufficient Mycobacterium tuberculosis antigens to stimulate immunity in genetically diverse human populations. We have used a novel approach to develop a synthetic scrambled antigen vaccine (TB-SAVINE), comprised of overlapping, recombined peptides from four M. tuberculosis proteins, Ag85B, ESAT-6, PstS3 and Mpt83, each of which is immunogenic and protective against experimental TB. This polyvalent TB-SAVINE construct stimulated CD4 and CD8T cell responses against the individual proteins and M. tuberculosis in C57BL/6 and Balb/c mice, when delivered as DNA, Fowl Pox Virus or Vaccinia Virus vaccines. In addition, the DNA-TBS vaccine induced protective immunity against pulmonary M. tuberculosis infection in C57BL/6 mice. Co-immunization of Balb/c mice with virally expressed TBS and HIV1-SAVINE vaccine stimulated strong T cell responses to both the M. tuberculosis and HIV proteins, indicating no effects of antigenic competition. Further development of this TB-SAVINE vaccine expressing components from multiple M. tuberculosis proteins may prove an effective vaccine candidate against TB, which could potentially form part of a safe, combined preventative strategy together with HIV immunisations.
Keyword Tuberculosis
Hiv
Bacille Calmette Guerin
Phosphate Transport Receptor
Mycobacterium Tuberculosis
Dna Vaccine
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 512269
NIAID AI-75320
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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