Immunological diversity within a family of cutinase-like proteins of Mycobacterium tuberculosis

West, Nicholas P., Wozniak, Teresa M., Valenzuela, Jesus, Feng, Carl G., Sher, Alan, Ribeiro, Jose M. C. and Britton, Warwick J. (2008) Immunological diversity within a family of cutinase-like proteins of Mycobacterium tuberculosis. Vaccine, 26 31: 3853-3859. doi:10.1016/j.vaccine.2008.05.007

Author West, Nicholas P.
Wozniak, Teresa M.
Valenzuela, Jesus
Feng, Carl G.
Sher, Alan
Ribeiro, Jose M. C.
Britton, Warwick J.
Title Immunological diversity within a family of cutinase-like proteins of Mycobacterium tuberculosis
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
Publication date 2008-07-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2008.05.007
Open Access Status Not yet assessed
Volume 26
Issue 31
Start page 3853
End page 3859
Total pages 7
Place of publication London, United Kingdom
Publisher Elsevier Ltd
Language eng
Formatted abstract
Secreted proteins of Mycobacterium tuberculosis play key roles in the assembly of the mycobacterial cell wall, with many being major targets of the host immune response. To date, meaningful characterization of a significant proportion of this important group of proteins is lacking. Among the group of putatively secreted proteins of M. tuberculosis are 7 cutinase-like proteins (CLP), not previously characterized in terms of their immunogenicity or vaccine protective efficacy. Although the CLP vary in the degree of homology with one another, they all share a similar active catalytic triad, closely homologous to that of the cutinase of Fusarium solani. By construction of DNA vaccines of all 7 CLP, and expression and purification of soluble, recombinant CLP, this study addresses the immunological responses to these proteins. Clp1, 2, 3 and 6 were found to elicit significant IFN-γ secretion in DNA immunized mice, with the antigens also demonstrating specificity in terms of CLP-generated T cell IFN-γ release, with minimal cross reactivity of humoral responses. Finally, following delivery of DNA vaccines, Clp1, 2 and 6, conferred a moderate yet reproducible and significant level of protection in a murine aerosol model of M. tuberculosis infection. 
Keyword Tuberculosis
Culture Filtrate Proteins
Protective Efficacy
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID Z01 AI000932-05
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 26 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 26 times in Scopus Article | Citations
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