Recovery of Ciguatoxin From Fish Flesh

Lewis, RJ and Sellin, M (1993) Recovery of Ciguatoxin From Fish Flesh. Toxicon, 31 10: 1333-1336. doi:10.1016/0041-0101(93)90407-A


Author Lewis, RJ
Sellin, M
Title Recovery of Ciguatoxin From Fish Flesh
Journal name Toxicon   Check publisher's open access policy
ISSN 0041-0101
Publication date 1993-10-01
Year available 1993
Sub-type Other
DOI 10.1016/0041-0101(93)90407-A
Open Access Status DOI
Volume 31
Issue 10
Start page 1333
End page 1336
Total pages 4
Place of publication OXFORD
Publisher PERGAMON-ELSEVIER SCIENCE LTD
Language eng
Abstract A mouse bioassay, validated for the quantification of ciguatoxin in up to 20 mg of ether extract from fish flesh, revealed that 63 +/- 14% of spiked ciguatoxin was recovered using a standard extraction procedure. Except for extracts from the least toxic of ciguateric fish (0.1-0.5 nmol ciguatoxin-1/kg fish), signs in mice of intoxication by ciguatoxin (hypothermia to below 33-degrees-C as well as at least severe diarrhoea or lachrymation or hypersalivation) could be distinguished from the toxic reaction that follows administration of ciguatoxin-free ether extracts. Ciguatoxin recovery was similar for four variants of the ether-water partition, with the 2M NaCl/ether partition extracting half the contaminants. The method described is selective for ciguatoxin and could be used to quantify natural levels ciguatoxin in the flesh of fish in the absence of a validated in vitro test.
Keyword Purification
Javanicus
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Other
Collection: ResearcherID Downloads - Archived
 
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