Rapid identification of primary constituents in parotoid gland secretions of the Australian cane toad using HPLC/MS-Q-TOF

Jing, Jing, Ren, Wei C., Li, Chun, Bose, Utpal, Parekh, Harendra S. and Wei, Ming Q. (2013) Rapid identification of primary constituents in parotoid gland secretions of the Australian cane toad using HPLC/MS-Q-TOF. Biomedical Chromatography, 27 6: 685-687. doi:10.1002/bmc.2858


Author Jing, Jing
Ren, Wei C.
Li, Chun
Bose, Utpal
Parekh, Harendra S.
Wei, Ming Q.
Title Rapid identification of primary constituents in parotoid gland secretions of the Australian cane toad using HPLC/MS-Q-TOF
Journal name Biomedical Chromatography   Check publisher's open access policy
ISSN 0269-3879
1099-0801
Publication date 2013-06-01
Sub-type Article (original research)
DOI 10.1002/bmc.2858
Volume 27
Issue 6
Start page 685
End page 687
Total pages 3
Place of publication Chichester, West Sussex, United Kingdom
Publisher John Wiley & Sons
Collection year 2014
Language eng
Abstract Toad parotoid gland secretion or toad venom has in recent years been increasingly shown to possess potentially beneficial pharmacological effects; this speculation has drawn much interest centred on elucidating the chemical basis of its multimodal effects. For this purpose, we explored the use of a rapid and accurate analysis method for systemic investigation of the parotoid gland chemistry, when extracted from Australian cane toads. Full-scan data of cane toad venom extract was acquired using high-performance liquid chromatography coupled with a hybrid quadrupole-time of flight mass spectrometry system (HPLC/MS-Q-TOF), with multiple ionization sources (ESI and APCI) in positive and negative mixed modes. By measuring the exact mass differences between the theoretical and measured mass of each assumed compound, we confirmed the presence of 12 key constituents. The present results demonstrate that the use of HPLC/MS-Q-TOF with multiple ionization sources delivers exemplary selectivity and sensitivity, allowing for the rapid and accurate identification of constituents within cane toad venom. This paves the way for this technique to be used in future routine screening of components within the genus Bufo and for key analytes too, then reliably assessed for any purported beneficial (clinic) properties.
Keyword Cane toad venom
Bufadienolides
HPLC
MS-Q-TOF
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Pharmacy Publications
 
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