A dynamic deep sleep stage in Drosophila

van Alphen, Bart, Yap, Melvyn H. W., Kirszenblat, Leonie, Kottler, Benjamin and van Swinderen, Bruno (2013) A dynamic deep sleep stage in Drosophila. Journal of Neuroscience, 33 16: 6917-6927. doi:10.1523/JNEUROSCI.0061-13.2013

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Author van Alphen, Bart
Yap, Melvyn H. W.
Kirszenblat, Leonie
Kottler, Benjamin
van Swinderen, Bruno
Title A dynamic deep sleep stage in Drosophila
Formatted title
A dynamic deep sleep stage in Drosophila
Journal name Journal of Neuroscience   Check publisher's open access policy
ISSN 0270-6474
1529-2401
Publication date 2013-04-01
Sub-type Article (original research)
DOI 10.1523/JNEUROSCI.0061-13.2013
Open Access Status File (Publisher version)
Volume 33
Issue 16
Start page 6917
End page 6927
Total pages 11
Place of publication Washington, DC, United States
Publisher Society for Neuroscience
Language eng
Abstract Howmight one determine whether simple animals such as flies sleep in stages? Sleep inmammalsis a dynamic process involving different stages of sleep intensity, and these are typically associated with measurable changes in brain activity (Blake and Gerard, 1937; Rechtschaffen and Kales, 1968; Webb and Agnew, 1971). Evidence for different sleep stages in invertebrates remains elusive, even though it has been well established that many invertebrate species require sleep (Campbell and Tobler, 1984; Hendricks et al., 2000; Shaw et al., 2000; Sauer et al., 2003). Here we used electrophysiology and arousal-testing paradigms to show that the fruit fly, Drosophila melanogaster, transitions between deeper and lighter sleep within extended bouts of inactivity, with deeper sleep intensities after15 and30 min of inactivity. As in mammals, the timing and intensity of these dynamic sleep processes in flies is homeostatically regulated and modulated by behavioral experience. Two molecules linked to synaptic plasticity regulate the intensity of the first deep sleep stage. Optogenetic upregulation of cyclic adenosine monophosphate during the day increases sleep intensity at night, whereas loss of function of a molecule involved in synaptic pruning, the fragile-X mental retardation protein, increases sleep intensity during the day. Our results show that sleep is not homogenous in insects, and suggest that waking behavior and the associated synaptic plasticity mechanisms determine the timing and intensity of deep sleep stages in Drosophila.
Keyword Mental-Retardation Protein
Synaptic Homeostasis
Brain Activity
Melanogaster
Need
Rest
Deprivation
Plasticity
Waking
Time
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2014 Collection
 
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