Synthesis and Assembly of Infectious Bovine Papillomavirus Particles Invitro

Zhou, J, Stenzel, DJ, Sun, XY and Frazer, IH (1993) Synthesis and Assembly of Infectious Bovine Papillomavirus Particles Invitro. Journal of General Virology, 74 763-768. doi:10.1099/0022-1317-74-4-763


Author Zhou, J
Stenzel, DJ
Sun, XY
Frazer, IH
Title Synthesis and Assembly of Infectious Bovine Papillomavirus Particles Invitro
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
Publication date 1993-04-01
Year available 1993
Sub-type Other
DOI 10.1099/0022-1317-74-4-763
Open Access Status Not yet assessed
Volume 74
Start page 763
End page 768
Total pages 6
Place of publication READING
Publisher SOC GENERAL MICROBIOLOGY
Language eng
Abstract Bovine papillomavirus type 1 (BPV-1) virions were produced in vitro using vaccinia virus (VV) recombinants expressing the BPV-1 L1 and L2 capsid proteins. Particles morphologically resembling papillomaviruses were observed in the nucleus of cells infected with a VV recombinant for the BPV-1 L1 protein, and greater numbers of similar particles were seen in the nuclei of cells infected with a VV double recombinant for L1 and L2. Virus-like particles (VLPs) assembled in cells infected with the VV double recombinant for BPV-1 L1 and L2, and not those assembled in cells infected with the VV recombinant for BPV-1 L1 alone, were able to package BPV-1 DNA. Transcription of the BPV-1 E1 viral open reading frame was observed after a mouse fibroblast cell line was exposed to VLPs produced using a BPV-1 L1/L2 VV recombinant in a cell line containing episomal BPV-1 DNA. E1 transcription was not observed when the VLPs were pre-incubated with antibodies to the capsid protein of BPV-1. This system should allow an in vitro approach to the definition of the BPV-1 cellular receptor.
Keyword Virus Dna
Type-1
Expression
Transformation
Sequences
Genome
Cells
L1
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Other
Collection: ResearcherID Downloads - Archived
 
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