Barrier screens: a method to sample blood-fed and host-seeking exophilic mosquitoes

Burkot, Thomas R., Russell, Tanya L., Reimer, Lisa J., Bugoro, Hugo, Beebe, Nigel W., Cooper, Robert D., Sukawati, Supraman, Collins, Frank H. and Lobo, Neil F. (2013) Barrier screens: a method to sample blood-fed and host-seeking exophilic mosquitoes. Malaria Journal, 12 1: 49.1-49.9. doi:10.1186/1475-2875-12-49


Author Burkot, Thomas R.
Russell, Tanya L.
Reimer, Lisa J.
Bugoro, Hugo
Beebe, Nigel W.
Cooper, Robert D.
Sukawati, Supraman
Collins, Frank H.
Lobo, Neil F.
Title Barrier screens: a method to sample blood-fed and host-seeking exophilic mosquitoes
Journal name Malaria Journal   Check publisher's open access policy
ISSN 1475-2875
Publication date 2013-02-01
Year available 2013
Sub-type Article (original research)
DOI 10.1186/1475-2875-12-49
Open Access Status DOI
Volume 12
Issue 1
Start page 49.1
End page 49.9
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Determining the proportion of blood meals on humans by outdoor-feeding and resting mosquitoes is challenging. This is largely due to the difficulty of finding an adequate and unbiased sample of resting, engorged mosquitoes to enable the identification of host blood meal sources. This is particularly difficult in the south-west Pacific countries of Indonesia, the Solomon Islands and Papua New Guinea where thick vegetation constitutes the primary resting sites for the exophilic mosquitoes that are the primary malaria and filariasis vectors.

Methods: Barrier screens of shade-cloth netting attached to bamboo poles were constructed between villages and likely areas where mosquitoes might seek blood meals or rest. Flying mosquitoes, obstructed by the barrier screens, would temporarily stop and could then be captured by aspiration at hourly intervals throughout the night.

Results: In the three countries where this method was evaluated, blood-fed females of Anopheles farauti, Anopheles bancroftii, Anopheles longirostris, Anopheles sundaicus, Anopheles vagus, Anopheles kochi, Anopheles annularis, Anopheles tessellatus, Culex vishnui, Culex quinquefasciatus and Mansonia spp were collected while resting on the barrier screens. In addition, female Anopheles punctulatus and Armigeres spp as well as male An. farauti, Cx. vishnui, Cx. quinquefasciatus and Aedes species were similarly captured.

Conclusions: Building barrier screens as temporary resting sites in areas where mosquitoes were likely to fly was an extremely time-effective method for collecting an unbiased representative sample of engorged mosquitoes for determining the human blood index.
Keyword Mosquito sampling
Human blood index
Barrier screen trap
Exophily
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 45114
SU19AI08986-03
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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