High-throughput DNA extraction from forest trees

Shepherd, Mervyn, Cross, Michael, Stokoe, Rhonda L., Scott, Leon J. and Jones, Megan E. (2002) High-throughput DNA extraction from forest trees. Plant Molecular Biology Reporter, 20 4: 425a-425j. doi:10.1007/BF02772134

Author Shepherd, Mervyn
Cross, Michael
Stokoe, Rhonda L.
Scott, Leon J.
Jones, Megan E.
Title High-throughput DNA extraction from forest trees
Journal name Plant Molecular Biology Reporter   Check publisher's open access policy
ISSN 0735-9640
Publication date 2002-12-01
Sub-type Article (original research)
DOI 10.1007/BF02772134
Volume 20
Issue 4
Start page 425a
End page 425j
Total pages 10
Place of publication New York, NY, United States
Publisher Springer
Language eng
Abstract It is difficult to extract pure high-quality DNA from trees, which may not be amenable to advances in extraction methods suitable for other plants. A new commercial high-throughput DNA extraction system, using a silica binding matrix for purification and a multisample mixer mill for tissue disruption, was evaluated for its suitability with Eucalyptus spp., Pinus spp., and Araucaria cunninghamii (hoop pine). DNA suitable for a range of molecular biology applications was successfully extracted from all genera. The method was highly reliable when tested in more than 500 preparations and could be adapted to different tree species with relatively minor modifications.
Keyword AFLP
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
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Created: Fri, 03 May 2013, 00:49:24 EST by Dr Megan Vance on behalf of School of Veterinary Science