A potential for overestimating the absolute magnitudes of second virial coefficients by small-angle X-ray scattering

Scott, David J., Patel, Trushar R. and Winzor, Donald J. (2013) A potential for overestimating the absolute magnitudes of second virial coefficients by small-angle X-ray scattering. Analytical Biochemistry, 435 2: 159-165. doi:10.1016/j.ab.2012.12.014


Author Scott, David J.
Patel, Trushar R.
Winzor, Donald J.
Title A potential for overestimating the absolute magnitudes of second virial coefficients by small-angle X-ray scattering
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
1096-0309
Publication date 2013-04-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.ab.2012.12.014
Volume 435
Issue 2
Start page 159
End page 165
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2014
Language eng
Abstract Theoretical consideration is given to the effect of cosolutes (including buffer and electrolyte components) on the determination of second virial coefficients for proteins by small-angle X-ray scattering (SAXS)—a factor overlooked in current analyses in terms of expressions for a two-component system. A potential deficiency of existing practices is illustrated by reassessment of published results on the effect of polyethylene glycol concentration on the second virial coefficient for urate oxidase. This error reflects the substitution of I(0,c3,0), the scattering intensity in the limit of zero scattering angle and solute concentration, for I(0,0,0), the corresponding parameter in the limit of zero cosolute concentration (c3) as well. Published static light scattering results on the dependence of the apparent molecular weight of ovalbumin on buffer concentration are extrapolated to zero concentration to obtain the true value (M2) and thereby establish the feasibility of obtaining the analogous SAXS parameter, I(0,0,0), experimentally.
Keyword Thermodynamic nonideality
Second virial coefficients
Small-angle X-ray scattering
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
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