Impact of Wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti

van den Hurk, Andrew F., Hall-Mendelin, Sonja, Pyke, Alyssa T., Frentiu, Francesca D., McElroy, Kate, Day, Andrew, Higgs, Stephen and O'Neill, Scott L. (2012) Impact of Wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti. PloS Neglected Tropical Diseases, 6 11: . doi:10.1371/journal.pntd.0001892


Author van den Hurk, Andrew F.
Hall-Mendelin, Sonja
Pyke, Alyssa T.
Frentiu, Francesca D.
McElroy, Kate
Day, Andrew
Higgs, Stephen
O'Neill, Scott L.
Title Impact of Wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti
Formatted title
Impact of Wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti
Journal name PloS Neglected Tropical Diseases   Check publisher's open access policy
ISSN 1935-2735
Publication date 2012-11-01
Year available 2012
Sub-type Article (original research)
DOI 10.1371/journal.pntd.0001892
Open Access Status DOI
Volume 6
Issue 11
Total pages 9
Place of publication San Francisco, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Incidence of disease due to dengue (DENV), chikungunya (CHIKV) and yellow fever (YFV) viruses is increasing in many parts of the world. The viruses are primarily transmitted by Aedes aegypti, a highly domesticated mosquito species that is notoriously difficult to control. When transinfected into Ae. aegypti, the intracellular bacterium Wolbachia has recently been shown to inhibit replication of DENVs, CHIKV, malaria parasites and filarial nematodes, providing a potentially powerful biocontrol strategy for human pathogens. Because the extent of pathogen reduction can be influenced by the strain of bacterium, we examined whether the wMel strain of Wolbachia influenced CHIKV and YFV infection in Ae. aegypti. Following exposure to viremic blood meals, CHIKV infection and dissemination rates were significantly reduced in mosquitoes with the wMel strain of Wolbachia compared to Wolbachia-uninfected controls. However, similar rates of infection and dissemination were observed in wMel infected and non-infected Ae. aegypti when intrathoracic inoculation was used to deliver virus. YFV infection, dissemination and replication were similar in wMel-infected and control mosquitoes following intrathoracic inoculations. In contrast, mosquitoes with the wMelPop strain of Wolbachia showed at least a 104 times reduction in YFV RNA copies compared to controls. The extent of reduction in virus infection depended on Wolbachia strain, titer and strain of the virus, and mode of exposure. Although originally proposed for dengue biocontrol, our results indicate a Wolbachia-based strategy also holds considerable promise for YFV and CHIKV suppression.
Keyword Monoclonal antibodies
Australian mosquitos
Dengue transmission
Virulent wolbachia
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article # e1892

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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