Nanog is an essential factor for induction of pluripotency in somatic cells from endangered felids

Verma,Rajneesh, Liu, Jun, Holland, Michael Kenneth, Temple-Smith, Peter, Williamson, Mark and Verma, Paul John (2013) Nanog is an essential factor for induction of pluripotency in somatic cells from endangered felids. BioResearch Open Access, 2 1: 72-76. doi:10.1089/biores.2012.0297

Author Verma,Rajneesh
Liu, Jun
Holland, Michael Kenneth
Temple-Smith, Peter
Williamson, Mark
Verma, Paul John
Title Nanog is an essential factor for induction of pluripotency in somatic cells from endangered felids
Journal name BioResearch Open Access   Check publisher's open access policy
ISSN 2164-7860
Publication date 2013-02
Year available 2013
Sub-type Article (original research)
DOI 10.1089/biores.2012.0297
Open Access Status DOI
Volume 2
Issue 1
Start page 72
End page 76
Total pages 5
Place of publication New Rochelle, NY,United States
Publisher Mary Ann Liebert
Collection year 2014
Language eng
Formatted abstract
Nanog has an important role in pluripotency induction in bovines and snow leopards. To examine whether it was
required for wild felids globally, we examined the induction of pluripotency in felids from Asia (Bengal tiger, Panthera
tigris), Africa (serval, Leptailurus serval), and the Americas (jaguar, Panthera onca). Dermal fibroblasts were
transduced with genes encoding the human transcription factors OCT4, SOX2, KLF4, and cMYC with or without
NANOG. Both four- and five-factor induction resulted in colony formation at day 3 in all three species tested;
however, we were not able to maintain colonies that were generated without NANOG beyond passage (P) 7.
Five-factor induced pluripotent stem cell (iPSC) colonies from wild cats were expanded in vitro on feeder layers
and were positive for alkaline phosphatase and protein expression of OCT-4, NANOG, and stage-specific embryonic
antigen-4 at P4 and P14. Reverse-transcription polymerase chain reaction confirmed that all five human
transgenes were transcribed at P4; however, OCT4, SOX2, and NANOG transgenes were silenced by P14. Endogenous
OCT4 and NANOG transcripts were detected at P4 and P14 in all cell lines confirming successful reprogramming.
At P14, the iPSCs from all three species remained euploid and differentiated in vivo and in vitro into
derivatives of the three germ layers. This study describes an effective method for inducing pluripotency in
three endangered wild cats from across the globe and confirms Nanog as an essential factor in the reprogramming
event. Efficient production of iPSC from endangered felids creates a unique opportunity for species preservation
through gamete production, nuclear transfer, embryo complementation, and future novel technologies.
Keyword Conservation
Iinduced pluripotent stem cells
Wild cats
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Online ahead of print January 22, 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Veterinary Science Publications
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Created: Fri, 12 Apr 2013, 17:04:39 EST by Dr Michael Holland on behalf of School of Veterinary Science