SIRT1 regulates the neurogenic potential of neural precursors in the adult subventricular zone and hippocampus

Saharan, Sumiti, Jhaveri, Dhanisha J. and Bartlett, Perry F. (2013) SIRT1 regulates the neurogenic potential of neural precursors in the adult subventricular zone and hippocampus. Journal of Neuroscience Research, 91 5: 642-659. doi:10.1002/jnr.23199

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
saharan_corrected_proof.pdf Full text (open access) application/pdf 986.93KB 0

Author Saharan, Sumiti
Jhaveri, Dhanisha J.
Bartlett, Perry F.
Title SIRT1 regulates the neurogenic potential of neural precursors in the adult subventricular zone and hippocampus
Journal name Journal of Neuroscience Research   Check publisher's open access policy
ISSN 0360-4012
Publication date 2013-05-01
Year available 2013
Sub-type Article (original research)
DOI 10.1002/jnr.23199
Open Access Status File (Author Post-print)
Volume 91
Issue 5
Start page 642
End page 659
Total pages 18
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract Within the two neurogenic niches of the adult mammalian brain, i.e., the subventricular zone lining the lateral ventricle and the subgranular zone of the hippocampus, there exist distinct populations of proliferating neural precursor cells that differentiate to generate new neurons. Numerous studies have suggested that epigenetic regulation by histone-modifying proteins is important in guiding precursor differentiation during development; however, the role of these proteins in regulating neural precursor activity in the adult neurogenic niches remains poorly understood. Here we examine the role of an NAD+-dependent histone deacetylase, SIRT1, in modulating the neurogenic potential of neural precursors in the neurogenic niches of the adult mouse brain. We show that SIRT1 is expressed by proliferating adult subventricular zone and hippocampal neural precursors, although its transcript and protein levels are dramatically reduced during neural precursor differentiation. Utilizing a lentiviral-mediated delivery strategy, we demonstrate that abrogation of SIRT1 signaling by RNAi does not affect neural precursor numbers or their proliferation. However, SIRT1 knock down results in a significant increase in neuronal production in both the subventricular zone and the hippocampus. In contrast, enhancing SIRT1 signaling either through lentiviral-mediated SIRT1 overexpression or through use of the SIRT1 chemical activator Resveratrol prevents adult neural precursors from differentiating into neurons. Importantly, knock down of SIRT1 in hippocampal precursors in vivo, either through RNAi or through genetic ablation, promotes their neurogenic potential. These findings highlight SIRT1 signaling as a negative regulator of neuronal differentiation of adult subventricular zone and hippocampal neural precursors.
Keyword Sirt1
Neural precursor cells
Adult neurogenesis
Neuronal differentiation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 13 February 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2014 Collection
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 40 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 42 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 07 Apr 2013, 11:07:20 EST by System User on behalf of Queensland Brain Institute