Diverse populations of lake water bacteria exhibit chemotaxis towards inorganic nutrients

Dennis, Paul G., Seymour, Justin, Kumbun, Kimber and Tyson, Gene W. (2013) Diverse populations of lake water bacteria exhibit chemotaxis towards inorganic nutrients. ISME Journal, 7 8: 1661-1664. doi:10.1038/ismej.2013.47


Author Dennis, Paul G.
Seymour, Justin
Kumbun, Kimber
Tyson, Gene W.
Title Diverse populations of lake water bacteria exhibit chemotaxis towards inorganic nutrients
Journal name ISME Journal   Check publisher's open access policy
ISSN 1751-7362
1751-7370
Publication date 2013-03-21
Year available 2013
Sub-type Article (original research)
DOI 10.1038/ismej.2013.47
Open Access Status Not yet assessed
Volume 7
Issue 8
Start page 1661
End page 1664
Total pages 4
Place of publication London, United Kingdom
Publisher Nature Publishing
Language eng
Subject 1105 Ecology, Evolution, Behavior and Systematics
2404 Microbiology
Abstract Chemotaxis allows microorganisms to rapidly respond to different environmental stimuli; however, understanding of this process is limited by conventional assays, which typically focus on the response of single axenic cultures to given compounds. In this study, we used a modified capillary assay coupled with flow cytometry and 16S rRNA gene amplicon pyrosequencing to enumerate and identify populations within a lake water microbial community that exhibited chemotaxis towards ammonium, nitrate and phosphate. All compounds elicited chemotactic responses from populations within the lake water, with members of Sphingobacteriales exhibiting the strongest responses to nitrate and phosphate, and representatives of the Variovorax, Actinobacteria ACK-M1 and Methylophilaceae exhibiting the strongest responses to ammonium. Our results suggest that chemotaxis towards inorganic substrates may influence the rates of biogeochemical processes.
Formatted abstract
Chemotaxis allows microorganisms to rapidly respond to different environmental stimuli; however, understanding of this process is limited by conventional assays, which typically focus on the response of single axenic cultures to given compounds. In this study, we used a modified capillary assay coupled with flow cytometry and 16S rRNA gene amplicon pyrosequencing to enumerate and identify populations within a lake water microbial community that exhibited chemotaxis towards ammonium, nitrate and phosphate. All compounds elicited chemotactic responses from populations within the lake water, with members of Sphingobacteriales exhibiting the strongest responses to nitrate and phosphate, and representatives of the Variovorax, Actinobacteria ACK-M1 and Methylophilaceae exhibiting the strongest responses to ammonium. Our results suggest that chemotaxis towards inorganic substrates may influence the rates of biogeochemical processes.
Keyword Chemotaxis
Microbial communities
16S rRNA gene
Inorganic substrates
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP1093175
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 28 Mar 2013, 19:40:48 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences