Development of defined culture conditions for expansion of human mesenchymal stromal cells for clinical applications

Frith, Jessica E., Jaramillo-Ferrada, Pamela, Mills, Richard J., Cameron, Andrew R., Titmarsh, Drew M., Wolvetang, Ernst J. and Cooper-White, Justin J. (2012). Development of defined culture conditions for expansion of human mesenchymal stromal cells for clinical applications. In M. A. Hayat (Ed.), Stem cells and cancer stem cells: therapeutic applications in disease and injury (pp. 13-26) Dordrecht, Netherlands: Springer Netherlands. doi:10.1007/978-94-007-4798-2_2


Author Frith, Jessica E.
Jaramillo-Ferrada, Pamela
Mills, Richard J.
Cameron, Andrew R.
Titmarsh, Drew M.
Wolvetang, Ernst J.
Cooper-White, Justin J.
Title of chapter Development of defined culture conditions for expansion of human mesenchymal stromal cells for clinical applications
Title of book Stem cells and cancer stem cells: therapeutic applications in disease and injury
Place of Publication Dordrecht, Netherlands
Publisher Springer Netherlands
Publication Year 2012
Sub-type Research book chapter (original research)
DOI 10.1007/978-94-007-4798-2_2
Open Access Status DOI
Series Stem Cells and Cancer Stem Cells
ISBN 9789400747975
9789400747982
Editor M. A. Hayat
Volume number 8
Chapter number 2
Start page 13
End page 26
Total pages 14
Total chapters 33
Language eng
Abstract/Summary Mesenchymal stromal cells (MSCs) are of clinical interest due to their ability to self-renew and differentiate into multiple tissue types. Unfortunately, both the number of MSCs within tissues and their reparative potential decreases with age, resulting in a decreased capability for tissue repair. The ability to isolate MSCs from a multitude of tissue sources and expand and differentiate them in vitro has made them excellent candidates for a range of therapeutic applications. However, a number of existing limitations in terms of their culture must first be addressed prior to them being widely available for use in the clinic. In this chapter we discuss potential strategies to produce therapeutically-relevant numbers of high-quality MSCs for use in clinical applications. The relative merits of MSCs derived from different tissue sources and the development of defined culture conditions, in terms of oxygen levels, soluble media and culture substrates from which high-quality MSCs may, in future be derived for clinical applications, are described.
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 27 Mar 2013, 01:22:31 EST by Dr Jessica Frith on behalf of Aust Institute for Bioengineering & Nanotechnology