Prevalence and molecular characterization of Enterobacteriaceae producing NDM-1 carbapenemase at a military hospital in Pakistan and evaluation of two chromogenic media

Day, Kathryn M., Ali, Shamshad, Mirza, Irfan Ali, Sidjabat, Hanna E., Silvey, Anna, Lanyon, Clare V., Cummings, Stephen P., Abbasi, Shahid Ahmed, Raza, Muhammad W., Paterson, David L. and Perry, John D. (2013) Prevalence and molecular characterization of Enterobacteriaceae producing NDM-1 carbapenemase at a military hospital in Pakistan and evaluation of two chromogenic media. Diagnostic Microbiology and Infectious Disease, 75 2: 187-191. doi:10.1016/j.diagmicrobio.2012.11.006


Author Day, Kathryn M.
Ali, Shamshad
Mirza, Irfan Ali
Sidjabat, Hanna E.
Silvey, Anna
Lanyon, Clare V.
Cummings, Stephen P.
Abbasi, Shahid Ahmed
Raza, Muhammad W.
Paterson, David L.
Perry, John D.
Title Prevalence and molecular characterization of Enterobacteriaceae producing NDM-1 carbapenemase at a military hospital in Pakistan and evaluation of two chromogenic media
Journal name Diagnostic Microbiology and Infectious Disease   Check publisher's open access policy
ISSN 0732-8893
1879-0070
Publication date 2013-02-01
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.diagmicrobio.2012.11.006
Open Access Status Not yet assessed
Volume 75
Issue 2
Start page 187
End page 191
Total pages 5
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Subject 2726 Microbiology (medical)
2725 Infectious Diseases
Abstract The aim of this study was to assess the frequency and genotypic diversity of carbapenemase-producing Enterobacteriaceae (CPE) in stool samples from patients attending a military hospital in Pakistan. Further aims included the identification of factors that might predispose to faecal carriage and evaluation of 2 chromogenic culture media: Brilliance CRE and chromID CARBA. Of 175 patients, 32 (183%) had faecal carriage of CPE and all produced NDM-1 carbapenemase. All of these 32 patients were detected using chromID CARBA compared with 20 patients (62.5%) detected using Brilliance CRE (P = 0.0015), Duration of hospitalization and treatment with co-amoxyclav were statistically associated with a higher likelihood of carriage of CPE (P <= 0.05). The majority of NDM-1-producing Enterobacteriaceae co-produced CTX-M-1 group extended spectrum beta-lactamase, and one third produced armA-type methylase. NDM-1 carbapenemase was most commonly found amongst commensal types of Escherichia coli, especially phylogenetic group B1. (C) 2013 Elsevier Inc. All rights reserved.
Formatted abstract
The aim of this study was to assess the frequency and genotypic diversity of carbapenemase-producing
Enterobacteriaceae (CPE) in stool samples from patients attending a military hospital in Pakistan. Further aims
included the identiļ¬cation of factors that might predispose to faecal carriage and evaluation of 2 chromogenic
culture media: Brilliance CRE and chromID CARBA. Of 175 patients, 32 (18.3%) had faecal carriage of CPE and all
produced NDM-1 carbapenemase. All of these 32 patients were detected using chromID CARBA compared with 20
patients (62.5%) detected using Brilliance CRE (P = 0.0015). Duration of hospitalization and treatment with coamoxyclav were statistically associated with a higher likelihood of carriage of CPE (P≤0.05). The majority of NDM-
1–producing Enterobacteriaceae co-produced CTX-M-1 group extended spectrum β-lactamase, and one third
produced armA-type methylase. NDM-1 carbapenemase was most commonly found amongst commensal types of
Escherichia coli, especially phylogenetic group B1.
Keyword Carbapenemase
Beta-lactamase
Enterobacteriaceae
Antimicrobial resistance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 14 December 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 17 Mar 2013, 11:20:12 EST by System User on behalf of UQ Centre for Clinical Research