Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo

Dancik, Yuri, Favre, Amandine, Loy, Chong Jin, Zvyagin, Andrei V. and Roberts, Michael S. (2013) Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo. Journal of Biomedical Optics, 18 2: . doi:10.1117/1.JBO.18.2.026022

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Author Dancik, Yuri
Favre, Amandine
Loy, Chong Jin
Zvyagin, Andrei V.
Roberts, Michael S.
Title Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo
Journal name Journal of Biomedical Optics   Check publisher's open access policy
ISSN 1083-3668
1560-2281
Publication date 2013-02-26
Year available 2013
Sub-type Article (original research)
DOI 10.1117/1.JBO.18.2.026022
Open Access Status File (Publisher version)
Volume 18
Issue 2
Total pages 13
Place of publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Language eng
Subject 2204 Biomedical Engineering
2502 Biomaterials
2504 Electronic, Optical and Magnetic Materials
3107 Atomic and Molecular Physics, and Optics
Abstract There is a growing body of literature showing the usefulness of multiphoton tomography (MPT) and fluorescence lifetime imaging for in situ characterization of skin constituents and the ensuing development of noninvasive diagnostic tools against skin diseases. Melanin and pigmentation-associated skin cancers constitute some of the major applications. We show that MPT and fluorescence lifetime imaging can be used to measure changes in cutaneous melanin concentration and that these can be related to the visible skin color. Melanin in the skin of African, Indian, Caucasian, and Asian volunteers is detected on the basis of its emission wavelength and fluorescence lifetimes in solution and in a melanocyte-keratinocyte cell culture. Fluorescence intensity is used to characterize the melanin content and distribution as a function of skin type and depth into the skin (stratum granulosum and stratum basale). The measured fluorescence intensities in given skin types agree with melanin amounts reported by others using biopsies. Our results suggest that spatial distribution of melanin in skin can be studied using MPT and fluorescence lifetime imaging, but further studies are needed to ascertain that the method can resolve melanin amount in smaller depth intervals.
Keyword biophotonics
fluorescence
multiphoton processes
tissues
Laser-Scanning Microscopy
2Nd-Harmonic Generation
2-Photon Microscopy
Ex-Vivo
Endogenous Fluorescence
Melanin Fluorescence
Clinical-Application
Beta-Lactoglobulin
Epidermal Melanin
Spectroscopy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes This article [J. Biomed. Opt.. 18, , 026022 (2013)] was originally published online on 14 February 2013 with an error in Fig. 7 caption. The word “corneum” has been replaced by “granulosum.” The corrected caption reads: Fig. 7 Maximum area-normalized fluorescence intensity corresponding to the lifetime τ1 in stratum granulosum and stratum basale versus mean L∗ value (Table 4) in (a) dorsal stratum granulosum; (b) dorsal stratum basale; (c) volar stratum granulosum; and (d) volar stratum basale. Data (mean±s:e:m:) from the five volunteers. This article was corrected online on 20 February 2013. It appears correctly in print

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
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