Rapid Discrimination of Echinococcus Species and Strains Using a Polymerase Chain Reaction-Based Rflp Method

Bowles, J and McManus, DP (1993) Rapid Discrimination of Echinococcus Species and Strains Using a Polymerase Chain Reaction-Based Rflp Method. Molecular and Biochemical Parasitology, 57 2: 231-240. doi:10.1016/0166-6851(93)90199-8


Author Bowles, J
McManus, DP
Title Rapid Discrimination of Echinococcus Species and Strains Using a Polymerase Chain Reaction-Based Rflp Method
Journal name Molecular and Biochemical Parasitology   Check publisher's open access policy
ISSN 0166-6851
Publication date 1993-02-01
Year available 1993
Sub-type Article (original research)
DOI 10.1016/0166-6851(93)90199-8
Open Access Status Not yet assessed
Volume 57
Issue 2
Start page 231
End page 240
Total pages 10
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Abstract Echinococcus species and genetically distinct strains of Echinococcus granulosus can be rapidly and reliably identified using a polymerase chain reaction (PCR)-linked restriction fragment length polymorphism (RFLP) method which surveys the sequence of a rapidly evolving region,of the ribosomal DNA (rDNA) unit. Internal transcribed spacer 1 (ITS1) of the rDNA repeat was amplified from various isolates and the product was digested with one of a number of 4-base cutting restriction enzymes. Characteristic patterns were produced when samples within various species and strain groups were analysed. This method offers an objective, simple, highly sensitive and rapid approach for the discrimination of Echinococcus isolates and for study of other parasite complexes.
Keyword Polymerase Chain Reaction
Restriction Fragment Length Polymorphism
Echinococcus
Ribosomal Dna
Strains
Dna
Granulosus
Sequences
Hosts
Genes
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID LP100200082
C-10-61H
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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