How specific is my SRM?: The issue of precursor and product ion redundancy

Sherman, Jamie, McKay, Matthew J., Ashman, Keith and Molloy, Mark P. (2009) How specific is my SRM?: The issue of precursor and product ion redundancy. Proteomics, 9 5: 1120-1123. doi:10.1002/pmic.200800577


Author Sherman, Jamie
McKay, Matthew J.
Ashman, Keith
Molloy, Mark P.
Title How specific is my SRM?: The issue of precursor and product ion redundancy
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9853
1615-9861
Publication date 2009-03-01
Year available 2009
Sub-type Article (original research)
DOI 10.1002/pmic.200800577
Open Access Status DOI
Volume 9
Issue 5
Start page 1120
End page 1123
Total pages 4
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag
Language eng
Formatted abstract
Abstract

Selected reaction monitoring (SRM) MS is proving to be a popular approach for targeted quantitative proteomics. The use of proteotypic peptides as candidates for SRM analysis is a wise first step in SRM method design. The obvious reason for this is the need to avoid redundancy at the sequence level, however this is incidental. The true reason is that homologous peptides result in redundancy in the mass-to-charge domain. This may seem like a trivial subtlety, however, we believe this is an issue of far greater significance than the proteomic community is aware. This VIEWPOINT article serves to highlight the complexity associated with designing SRM assays in light of potential ion redundancy.
Keyword Mass spectrometry
MRM
Peptide identification
Redundancy
SRM
Reaction Monitoring Assays
Absolute Quantification
Plasma-Proteins
Mass-Spectrometry
High-Sensitivity
Multiple
Peptides
Serum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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