Phosphoproteomics and cancer research

Ashman, Keith and Lopez Villar, Elena (2009) Phosphoproteomics and cancer research. Clinical and Translational Oncology, 11 6: 356-362. doi:10.1007/s12094-009-0369-z


Author Ashman, Keith
Lopez Villar, Elena
Title Phosphoproteomics and cancer research
Journal name Clinical and Translational Oncology   Check publisher's open access policy
ISSN 1699-048X
1699-3055
1699-5384
Publication date 2009-06-01
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s12094-009-0369-z
Volume 11
Issue 6
Start page 356
End page 362
Total pages 7
Place of publication Milan, Italy
Publisher Springer Italia
Language eng
Formatted abstract
Abstract

Protein phosphorylation plays key roles in the regulation of normal and cancer cells. It is a highly dynamic process. Protein kinases are the targets of several new cancer drugs and drug candidates. However, some of the main issues related to new drugs are how they function and the selection of those patients that will likely respond best to a particular treatment regime. There is an urgent need to understand and monitor kinase signalling pathways. Phosphoproteomics requires the enrichment of phosphorylated proteins or peptides from tissue or bodily fluids, and the application of technologies such as mass spectrometry (MS) to the identification and quantification of protein phosphorylation sites. As the field develops it will provide pharmacodynamic readouts of disease states and cellular drug responses in tumour samples. There have been a number of recent advances, but there are still technical hurdles and bioinformatics challenges that need to be addressed.

Keyword Immobilised metal ion affinity chromatography (IMAC)
Titanium dioxide
Mass spectrometry (MS)
Protein phosphorylation
Kinase signalling
Cancer
Multiply Phosphorylated Peptides
Quantitative Proteomic Analysis
Ion Affinity-Chromatography
Stained Polyacrylamide-Gels
Mass-Spectrometric Analysis
Tyrosine Kinase Inhibitors
Posttranslational Modifications
Signaling Networks
In-Vivo
Phosphopeptide Enrichment
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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