Peptidomic profiles of post myocardial infarction rats affinity depleted plasma using matrix-assisted laser desorption/ionization time of flight (MALDI-ToF) mass spectrometry

Wang, Bing Hui, Reisman, Simone, Bailey, Mark, Kompa, Andrew, Ayhan, Mustafa, Krum, Henry and Rice, Gregory (2012) Peptidomic profiles of post myocardial infarction rats affinity depleted plasma using matrix-assisted laser desorption/ionization time of flight (MALDI-ToF) mass spectrometry. Clinical and Translational Medicine, 1 11.1-11.8. doi:10.1186/2001-1326-1-11

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Author Wang, Bing Hui
Reisman, Simone
Bailey, Mark
Kompa, Andrew
Ayhan, Mustafa
Krum, Henry
Rice, Gregory
Title Peptidomic profiles of post myocardial infarction rats affinity depleted plasma using matrix-assisted laser desorption/ionization time of flight (MALDI-ToF) mass spectrometry
Journal name Clinical and Translational Medicine   Check publisher's open access policy
ISSN 2001-1326
Publication date 2012-01-01
Year available 2012
Sub-type Article (original research)
DOI 10.1186/2001-1326-1-11
Open Access Status DOI
Volume 1
Start page 11.1
End page 11.8
Total pages 8
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Background: Despite major advances in drug development, effective cardiovascular therapies and suitable cardiovascular biomarkers remain limited. The aim of this study was to leverage mass spectrometry (MS) based peptide profiling strategies to identify changes that occur in peptidomic profiles of rat plasma following coronary artery ligation generated myocardial infarction (MI).

Methods: One week after MI, rats were randomized to receive either an ACE inhibitor (ramipril, Ram-1 mg/kg/day), or vehicle (Veh) for 12 weeks. Echocardiography and hemodynamic measurements were made before sacrifice and plasma collection. High abundance proteins were depleted with affinity capture before MS profiling. Differentially
expressed peptide ions were identified using proprietary software (ClinProtTools).

Results: MI increased heart/body weight (18%), lung/body weight (56%), and left ventricular (LV) end diastolic pressure (LVEDP, 247%); and significantly reduced percentage fractional shortening (FS, 75%) and rate of pressure rise in the LV (dP/dtmax, 20%). Ram treatment significantly attenuated the changes in LVEDP (61%) and FS (27%). Analysis of MALDI-ToF generated mass spectra demonstrated that peptide ions 1271, 1878, 1955, 2041 and 2254 m/z were consistently decreased by Ram treatment (p<0.001) and thus may be associated with the agent’s therapeutic  effects. Among peptides that were significantly changed, synapsin-2, adenomatous polyposis coli protein and transcription factor jun-D were identified as significantly reduced by Ram treatment.

Conclusions: This approach allows us to screen for potential biomarkers in a window of the blood proteome that previously has been difficult to access. The data obtained from such an approach may potentially useful in prognosis, diagnosis, and monitoring of treatment response.
Keyword Myocardial infarction
Peptidomic profiling
Mass spectrometry
Biomarkers
Heart failure
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article 11

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
 
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Created: Thu, 14 Feb 2013, 21:09:59 EST by Mrs Maureen Pollard on behalf of Paediatrics & Child Health - RBWH