Characterisation of antibody responses in pigs induced by recombinant oncosphere antigens from Taenia solium

Jayashi, Cesar M., Gonzalez, Armando E., Neyra, Ricardo Castillo, Kyngdon, Craig T., Gauci, Charles G. and Lightowlers, Marshall W. (2012) Characterisation of antibody responses in pigs induced by recombinant oncosphere antigens from Taenia solium. Vaccine, 30 52: 7475-7480. doi:10.1016/j.vaccine.2012.10.057


Author Jayashi, Cesar M.
Gonzalez, Armando E.
Neyra, Ricardo Castillo
Kyngdon, Craig T.
Gauci, Charles G.
Lightowlers, Marshall W.
Title Characterisation of antibody responses in pigs induced by recombinant oncosphere antigens from Taenia solium
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
1873-2518
Publication date 2012-12-01
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2012.10.057
Volume 30
Issue 52
Start page 7475
End page 7480
Total pages 6
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract
Recombinant antigens cloned from the oncosphere life cycle stage of the cestode parasite Taenia solium (T. solium) have been proven to be effective as vaccines for protecting pigs against infections with T. solium. Previous studies have defined three different host protective oncosphere antigens, TSOL18, TSOL16 and TSOL45. In this study, we evaluated the potential for combining the antigens TSOL16 and TSOL18 as a practical vaccine. Firstly, in a laboratory trial, we compared the immunogenicity of the combined antigens (TSOL16/18) versus the immunogenicity of the antigens separately. Secondly, in a field trial, we tested the ability of the TSOL16/18 vaccine to induce detectable antibody responses in animals living under environmental stress and traditionally reared in areas where T. solium cysticercosis is endemic; and finally, we characterised the immune response of the study population. Pigs of 8-16 weeks of age were vaccinated with 200μg each of TSOL16 and TSOL18, plus 5mg of Quil-A. Specific total IgG, IgG1 and IgG2 antibody responses induced by TSOL16 and TSOL18 were determined with ELISA. The immunogenicity of both antigens was retained in the combined TSOL16/18 vaccine. The combined vaccine TSOL16/18 induced detectable specific anti-TSOL18 antibody responses in 100% (113/113) and specific anti-TSOL16 in 99% (112/113) of the vaccinated animals measured at 2 weeks following the booster vaccination. From the two IgG antibody subtypes analysed we found there was stronger response to IgG2.
Keyword Pigs
Cysticercosis
Neurocysticercosis
Vaccination
Oncosphere
T. solium
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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