A simple method for fabricating 3-D multilayered composite scaffolds

Vaquette, Cedryck and Cooper-White, Justin (2013) A simple method for fabricating 3-D multilayered composite scaffolds. Acta Biomaterialia, 9 1: 4599-4608. doi:10.1016/j.actbio.2012.08.015

Author Vaquette, Cedryck
Cooper-White, Justin
Title A simple method for fabricating 3-D multilayered composite scaffolds
Journal name Acta Biomaterialia   Check publisher's open access policy
ISSN 1742-7061
Publication date 2013-01-01
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.actbio.2012.08.015
Open Access Status Not yet assessed
Volume 9
Issue 1
Start page 4599
End page 4608
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract One limitation of electrospinning stems from the charge build-up that occurs during processing, preventing further fibre deposition and limiting the scaffold overall thickness and hence their end-use in tissue engineering applications targeting large tissue defect repair. To overcome this, we have developed a technique in which thermally induced phase separation (TIPS) and electrospinning are combined. Thick three-dimensional, multilayered composite scaffolds were produced by simply stacking individual polycaprolactone (PCL) microfibrous electrospun discs into a cylindrical holder that was filled with a 3% poly(lactic-co-glycolic acid) (PLGA) solution in dimethylsulfoxide (a good solvent for PLGA but a poor one for PCL). The construct was quenched in liquid nitrogen and the solvent removed by leaching out in cold water. This technique enables the fabrication of scaffolds composed principally of electrospun membranes that have no limit to their thickness. The mechanical properties of these scaffolds were assessed under both quasi-static and dynamic conditions. The multilayered composite scaffolds had similar compressive properties to 5% PCL scaffolds fabricated solely by the TIPS methodology. However, tensile tests demonstrated that the multilayered construct outperformed a scaffold made purely by TIPS, highlighting the contribution of the electrospun component of the composite scaffold to enhancing the overall mechanical property slate. Cell studies revealed cell infiltration principally from the scaffold edges under static seeding conditions. This fabrication methodology permits the rapid construction of thick, strong scaffolds from a range of biodegradable polymers often used in tissue engineering, and will be particularly useful when large dimension electrospun scaffolds are required.
Keyword Electrospinning
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 16 August 2012.

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