Effect of long-term starvation conditions on polyphosphate- and glycogen-accumulating organisms

Vargas, M., Yuan, Z. and Pijuan, M. (2013) Effect of long-term starvation conditions on polyphosphate- and glycogen-accumulating organisms. Bioresource Technology, 127 126-131. doi:10.1016/j.biortech.2012.09.117

Author Vargas, M.
Yuan, Z.
Pijuan, M.
Title Effect of long-term starvation conditions on polyphosphate- and glycogen-accumulating organisms
Journal name Bioresource Technology   Check publisher's open access policy
ISSN 0960-8524
Publication date 2013-01-01
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.biortech.2012.09.117
Open Access Status DOI
Volume 127
Start page 126
End page 131
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Measurements of historical specimens are widely applied in studies of taxonomy, systematics, and ecology, but biologists often assume that the effects of preservative chemicals on the morphology of amphibian specimens are minimal in their analyses. We compared the body length and body mass of 182 samples of 13 live and preserved (up to 10 years) anuran species and found that the body length and body mass of preserved specimens significantly decreased by 6.1% and 24.8%, respectively, compared to those measurements of their live counterparts. The changes in body length and mass also exhibited highly significant variations between species. Similarly, there were significant differences in shrinkage of body length and body mass between sexes, where males showed greater shrinkage in body length and body mass compared to females. Preservation distorted the magnitude of the interspecific differences in body length observed in the fresh specimens. Overall, the reduction in body length or mass was greater in longer or heavier individuals. Due to the effects of preservation on amphibian morphology, we propose two parsimonious conversion equations to back-calculate the original body length and body mass of studied anurans for researchers working with historical data, since morphological data from preserved specimens may lead to incorrect biological interpretations when comparing to fresh specimens. Therefore, researchers should correct for errors due to preservation effects that may lead to the misinterpretation of results.
Formatted abstract
Endogenous processes such as biomass decay and intracellular polymers degradation of polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs) were investigated. Cultures enriched in Accumulibacter (a well known PAO) or Competibacter (a well known GAO) were subjected to 21 and 26days of alternating anaerobic/aerobic conditions respectively. The main energy source for PAOs during starvation was their intracellular polyphosphate released into the medium during the first 14days of starvation. In contrast, GAOs used their intracellular glycogen during the 26days of starvation. Biomass decay rates were 0.029d-1 for PAOs and almost negligible for GAOs. The reduction in acetate uptake rate during the starvation period, referred to as activity decay, was 0.25 and 0.047d-1 for PAOs and GAOs, respectively. Once wastewater was reintroduced, both populations recovered their initial substrate uptake rate after 1day. The results obtained show that PAOs are more affected than GAOs by starvation conditions.
Keyword Polyphosphate-accumulating organisms
Glycogen-accumulating organisms
Endogenous metabolism
Starvation conditions
Decay processes
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 2008-BE2-00244
Institutional Status UQ
Additional Notes Available online: 10 October 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
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