Analysis of the extreme diversity of salivary alpha-amylase isoforms generated by physiological proteolysis using liquid chromatography-tandem mass spectrometry

Bailey, Ulla-Maja, Punyadeera, Chamindie, Cooper-White, Justin J. and Schulz, Benjamin L. (2012) Analysis of the extreme diversity of salivary alpha-amylase isoforms generated by physiological proteolysis using liquid chromatography-tandem mass spectrometry. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 911 21-26. doi:10.1016/j.jchromb.2012.10.023

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Author Bailey, Ulla-Maja
Punyadeera, Chamindie
Cooper-White, Justin J.
Schulz, Benjamin L.
Title Analysis of the extreme diversity of salivary alpha-amylase isoforms generated by physiological proteolysis using liquid chromatography-tandem mass spectrometry
Journal name Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences   Check publisher's open access policy
ISSN 1570-0232
1873-376X
Publication date 2012-12-01
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.jchromb.2012.10.023
Open Access Status File (Author Post-print)
Volume 911
Start page 21
End page 26
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 1602 Analytical Chemistry
1303 Biochemistry
1308 Clinical Biochemistry
1307 Cell Biology
Abstract Saliva is a crucial biofluid for oral health and is also of increasing importance as a non-invasive source of disease biomarkers. Salivary alpha-amylase is an abundant protein in saliva, and changes in amylase expression have been previously associated with a variety of diseases and conditions. Salivary alpha-amylase is subject to a high diversity of post-translational modifications, including physiological proteolysis in the oral cavity. Here we developed methodology for rapid sample preparation and non-targeted LC-ESI-MS/MS analysis of saliva from healthy subjects and observed an extreme diversity of alpha-amylase proteolytic isoforms. Our results emphasize the importance of consideration of post-translational events such as proteolysis in proteomic studies, biomarker discovery and validation, particularly in saliva.
Keyword Saliva
Alpha-amylase
Liquid chromatography
Mass spectrometry
Proteolysis
Biomarker
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 631615
APP1031542
Institutional Status UQ
Additional Notes Available online: 26 October 2012. NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography B. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B, 911 2013 DOI:10.1016/j.jchromb.2012.10.023

 
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