Effects of breaking up prolonged sitting on skeletal muscle gene expression

Latouche, Celine, Jowett, Jeremy B. M., Carey, Andrew L., Bertovic, David A., Owen, Neville, Dunstan, David W. and Kingwell, Bronwyn A. (2013) Effects of breaking up prolonged sitting on skeletal muscle gene expression. Journal of Applied Physiology, 114 4: 453-460. doi:10.1152/japplphysiol.00978.2012

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Author Latouche, Celine
Jowett, Jeremy B. M.
Carey, Andrew L.
Bertovic, David A.
Owen, Neville
Dunstan, David W.
Kingwell, Bronwyn A.
Title Effects of breaking up prolonged sitting on skeletal muscle gene expression
Journal name Journal of Applied Physiology   Check publisher's open access policy
ISSN 8750-7587
1522-1601
Publication date 2013-02-15
Year available 2012
Sub-type Article (original research)
DOI 10.1152/japplphysiol.00978.2012
Volume 114
Issue 4
Start page 453
End page 460
Total pages 8
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Collection year 2013
Language eng
Abstract Breaking up prolonged sitting has been beneficially associated with cardiometabolic risk markers in both observational and intervention studies. We aimed to define the acute transcriptional events induced in skeletal muscle by breaks in sedentary time. Overweight/obese adults participated in a randomized three-period, three-treatment crossover trial in an acute setting. The three 5-h interventions were performed in the postprandial state after a standardized test drink and included seated position with no activity and seated with 2-min bouts of light- or moderate-intensity treadmill walking every 20 min. Vastus lateralis biopsies were obtained in eight participants after each treatment, and gene expression was examined using microarrays validated with real-time quantitative PCR. There were 75 differentially expressed genes between the three conditions. Pathway analysis indicated the main biological functions affected were related to small-molecule biochemistry, cellular development, growth and proliferation, and carbohydrate metabolism. Interestingly, differentially expressed genes were also linked to cardiovascular disease. For example, relative to prolonged sitting, activity bouts increased expression of nicotamide N-methyltransferase, which modulates anti-inflammatory and anti-oxidative pathways and triglyceride metabolism. Activity bouts also altered expression of 10 genes involved in carbohydrate metabolism, including increased expression of dynein light chain, which may regulate translocation of the GLUT-4 glucose transporter. In addition, breaking up sedentary time reversed the effects of chronic inactivity on expression of some specific genes. This study provides insight into the muscle regulatory systems and molecular processes underlying the physiological benefits induced by interrupting prolonged sitting.
Keyword Sedentary
Exercise
Microarray
Metabolism
Intermittent
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published online December 27, 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Public Health Publications
 
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Created: Thu, 17 Jan 2013, 01:48:41 EST by Geraldine Fitzgerald on behalf of School of Public Health