Unique and Cross-Reactive T Cell Epitope Peptides of the Major Bahia Grass Pollen Allergen, Pas n 1

Etto, Tamara, de Boer, Carmela, Prickett, Sara, Gardner, Leanne M., Voskamp, Astrid, Davies, Janet M., O'Hehir, Robyn E. and Rolland, Jennifer M. (2012) Unique and Cross-Reactive T Cell Epitope Peptides of the Major Bahia Grass Pollen Allergen, Pas n 1. International Archives of Allergy and Immunology, 159 4: 355-366. doi:10.1159/000338290


Author Etto, Tamara
de Boer, Carmela
Prickett, Sara
Gardner, Leanne M.
Voskamp, Astrid
Davies, Janet M.
O'Hehir, Robyn E.
Rolland, Jennifer M.
Title Unique and Cross-Reactive T Cell Epitope Peptides of the Major Bahia Grass Pollen Allergen, Pas n 1
Journal name International Archives of Allergy and Immunology   Check publisher's open access policy
ISSN 1018-2438
1423-0097
Publication date 2012-01-01
Sub-type Article (original research)
DOI 10.1159/000338290
Volume 159
Issue 4
Start page 355
End page 366
Total pages 12
Place of publication Basel, Switzerland
Publisher S. Karger
Language eng
Formatted abstract
Background: Bahia grass pollen (BaGP) is a major cause of allergic rhinitis. Subcutaneous allergen-specific immunotherapy is effective for grass pollen allergy, but is unsuitable for patients with moderate to severe asthma due to the risk of anaphylaxis. T cell-reactive but IgE nonreactive peptides provide a safer treatment option. This study aimed to identify and characterize dominant CD4 + T cell epitope peptides of the major BaGP allergen, Pas n 1.
Methods: Pas n 1-specific T cell lines generated from the peripheral blood of BaGP-allergic subjects were tested for proliferative and cytokine response to overlapping 20-mer Pas n 1 peptides. Cross-reactivity to homologous peptides from Lol p 1 and Cyn d 1 of Ryegrass and Bermuda grass pollen, respectively, was assessed using Pas n 1 peptide-specific T cell clones. MHC class II restriction of Pas n 1 peptide T cell recognition was determined by HLA blocking assays and peptide IgE reactivity tested by dot blotting. Results: Three Pas n 1 peptides showed dominant T cell reactivity; 15 of 18 (83%) patients responded to one or more of these peptides. T  cell clones specific for dominant Pas n 1 peptides showed evidence of species-specific T cell reactivity as well as cross-reactivity with other group 1 grass pollen allergens. The dominant Pas n 1 T cell epitope peptides showed HLA binding diversity and were non-IgE reactive.
Conclusions: The immunodominant T cell-reactive Pas n 1 peptides are candidates for safe immunotherapy for individuals, including those with asthma, who are allergic to Bahia and possibly other grass pollens.
Keyword T cell epitopes
T cell cross-reactivity
Bahia grass pollen allergy
Allergic rhinitis
Seasonal Rhinitis
Hla-Dr
Immunotherapy
Asthma
Responses
Clones
Rhinoconjunctivitis
Expression
Induction
Tolerance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
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