A novel type III endosome transmembrane protein, TEMP

Aturaliya, Rajith, Kerr, Markus and Teasdale, Rohan D. (2012) A novel type III endosome transmembrane protein, TEMP. Cells, 1 4: 1029-1044. doi:10.3390/cells1041029

Author Aturaliya, Rajith
Kerr, Markus
Teasdale, Rohan D.
Title A novel type III endosome transmembrane protein, TEMP
Journal name Cells   Check publisher's open access policy
ISSN 2073-4409
Publication date 2012-11-05
Sub-type Article (original research)
DOI 10.3390/cells1041029
Open Access Status DOI
Volume 1
Issue 4
Start page 1029
End page 1044
Total pages 16
Place of publication Basel, Switzerland
Publisher M D P I AG
Collection year 2013
Language eng
Abstract As part of a high-throughput subcellular localisation project, the protein encoded by the RIKEN mouse cDNA 2610528J11 was expressed and identified to be associated with both endosomes and the plasma membrane. Based on this, we have assigned the name TEMP for Type III Endosome Membrane Protein. TEMP encodes a short protein of 111 amino acids with a single, alpha-helical transmembrane domain. Experimental analysis of its membrane topology demonstrated it is a Type III membrane protein with the amino-terminus in the lumenal, or extracellular region, and the carboxy-terminus in the cytoplasm. In addition to the plasma membrane TEMP was localized to Rab5 positive early endosomes, Rab5/Rab11 positive recycling endosomes but not Rab7 positive late endosomes. Video microscopy in living cells confirmed TEMP's plasma membrane localization and identified the intracellular endosome compartments to be tubulovesicular. Overexpression of TEMP resulted in the early/recycling endosomes clustering at the cell periphery that was dependent on the presence of intact microtubules. The cellular function of TEMP cannot be inferred based on bioinformatics comparison, but its cellular distribution between early/recycling endosomes and the plasma membrane suggests a role in membrane transport.
Keyword Endoscopes
Subcellular localisation
Membraine protein
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Institute for Molecular Bioscience - Publications
Official 2013 Collection
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Created: Fri, 14 Dec 2012, 10:45:27 EST by Susan Allen on behalf of Institute for Molecular Bioscience