The aversive phototaxic suppression assay for individual adult Drosophila

van Swinderen. Bruno (2011) The aversive phototaxic suppression assay for individual adult Drosophila. Cold Spring Harbor Protocols, 6 10: 1203-1205. doi:10.1101/pdb.prot065896


Author van Swinderen. Bruno
Title The aversive phototaxic suppression assay for individual adult Drosophila
Formatted title
The aversive phototaxic suppression assay for individual adult Drosophila
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1940-3402
1559-6095
Publication date 2011-10-01
Sub-type Article (original research)
DOI 10.1101/pdb.prot065896
Volume 6
Issue 10
Start page 1203
End page 1205
Total pages 3
Place of publication Woodbury, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Formatted abstract
Vision is a major sensory modality in Drosophila behavior, with more than one-half of the Drosophila brain devoted to visual processing. The mechanisms of vision in Drosophila can now be studied in individuals and in populations of flies by using various paradigms. Simple strategies for conducting visual perception and learning studies consist of individual studies performed on single flies on solid supports (larvae on agar or adults in a T-maze) using a light/dark association paradigm. These approaches are quite easy to implement but are fairly limited in their ability to address questions of visual perception. Nevertheless, the simpler approaches treating vision in one dimension (light, dark) do provide effective paradigms for genetic analysis. For adult flies, a paradigm called aversive phototaxic suppression (APS), as described here, can be used. This method exploits flies’ phototaxic reflex. By associating a lit chamber with quinine (which is aversive), repeated trials on a single animal result in a learned response to avoid (or to suppress) phototaxis. Of note, the unconditioned stimulus (US) quinine must be present throughout the experiment for APS to work, unlike other memory assays in which the US is removed during testing.
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Fri, 07 Dec 2012, 22:44:33 EST by Debra McMurtrie on behalf of Queensland Brain Institute