An assay for visual learning in individual Drosophila larvae

van Swinderen, Bruno (2011) An assay for visual learning in individual Drosophila larvae. Cold Spring Harbor Protocols, 6 10: 1200-1202. doi:10.1101/pdb.prot065888

Author van Swinderen, Bruno
Title An assay for visual learning in individual Drosophila larvae
Formatted title
An assay for visual learning in individual Drosophila larvae
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1940-3402
Publication date 2011-10-01
Sub-type Article (original research)
DOI 10.1101/pdb.prot065888
Open Access Status Not yet assessed
Volume 6
Issue 10
Start page 1200
End page 1202
Total pages 3
Place of publication Woodbury, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Formatted abstract
Vision is a major sensory modality in Drosophila behavior, with more than one-half of the Drosophila brain devoted to visual processing. The mechanisms of vision in Drosophila can now be studied in individuals and in populations of flies by using various paradigms. Simple strategies for conducting visual perception and learning studies consist of individual studies performed on single flies on solid supports (larvae on agar or adults in a T-maze) using a light/dark association paradigm. These approaches are quite easy to implement but are fairly limited in their ability to address questions of visual perception. Nevertheless, the simpler approaches treating vision in one dimension (light, dark) do provide effective paradigms for genetic analysis. This article describes a protocol for larval visual learning. Larvae are transferred back and forth between well-lit or dark agarose plates that either do or do not contain fructose (which is appetitive) as the unconditioned stimulus (US). Such appetitive larval visual memory is tested by tallying the time spent in light or dark quadrants of a plate (without a US).
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
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Citation counts: Scopus Citation Count Cited 2 times in Scopus Article | Citations
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Created: Fri, 07 Dec 2012, 22:33:35 EST by Debra McMurtrie on behalf of Queensland Brain Institute