The family of Deg/HtrA proteases in plants

Schuhmann, Holger, Huesgen, Pitter F. and Adamska, Iwona (2012) The family of Deg/HtrA proteases in plants. BMC Plant Biology, 12 52.1-52.14. doi:10.1186/1471-2229-12-52

Author Schuhmann, Holger
Huesgen, Pitter F.
Adamska, Iwona
Title The family of Deg/HtrA proteases in plants
Journal name BMC Plant Biology   Check publisher's open access policy
ISSN 1471-2229
Publication date 2012-04-01
Sub-type Article (original research)
DOI 10.1186/1471-2229-12-52
Open Access Status DOI
Volume 12
Start page 52.1
End page 52.14
Total pages 14
Place of publication BioMed Central
Publisher London, United Kingdom
Language eng
Formatted abstract
The Deg/HtrA family of ATP-independent serine endopeptidases is present in nearly all organisms from bacteria to human and vascular plants. In recent years, multiple deg/htrA protease genes were identified in various plant genomes. During genome annotations most proteases were named according to the order of discovery, hence the same names were sometimes given to different types of Deg/HtrA enzymes in different plant species. This can easily lead to false inference of individual protease functions based solely on a shared name. Therefore, the existing names and classification of these proteolytic enzymes does not meet our current needs and a phylogeny-based standardized nomenclature is required.

Using phylogenetic and domain arrangement analysis, we improved the nomenclature of the Deg/HtrA protease family, standardized protease names based on their well-established nomenclature in Arabidopsis thaliana, and clarified the evolutionary relationship between orthologous enzymes from various photosynthetic organisms across several divergent systematic groups, including dicots, a monocot, a moss and a green alga. Furthermore, we identified a “core set” of eight proteases shared by all organisms examined here that might provide all the proteolytic potential of Deg/HtrA proteases necessary for a hypothetical plant cell.


In our proposed nomenclature, the evolutionarily closest orthologs have the same protease name, simplifying scientific communication when comparing different plant species and allowing for more reliable inference of protease functions. Further, we proposed that the high number of Deg/HtrA proteases in plants is mainly due to gene duplications unique to the respective organism.
Keyword Multiple sequence alignment
Thylakoid membrane
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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