Assessment of pathogen survival potential during managed aquifer recharge with diffusion chambers

Sidhu, J. P. S. and Toze, S. (2012) Assessment of pathogen survival potential during managed aquifer recharge with diffusion chambers. Journal of Applied Microbiology, 113 3: 693-700. doi:10.1111/j.1365-2672.2012.05360.x

Author Sidhu, J. P. S.
Toze, S.
Title Assessment of pathogen survival potential during managed aquifer recharge with diffusion chambers
Journal name Journal of Applied Microbiology   Check publisher's open access policy
ISSN 1364-5072
Publication date 2012-09-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2672.2012.05360.x
Open Access Status Not yet assessed
Volume 113
Issue 3
Start page 693
End page 700
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Formatted abstract
Aims: To evaluate the efficacy of using in situ diffusion chambers for pathogen decay studies in the aquifer.
Methods and Results: A comparative microbial inactivation study was carried out in groundwater by seeding selected pathogens and indicators in laboratory microcosms and Teflon diffusion chambers (in situ) fitted with 0.010- and 0.025-μm pore-size membranes. The results have shown that there is a difference in the decay rates obtained from the laboratory microcosms and in situ diffusion chambers for several pathogens. The results suggest that the use of laboratory microcosms to determine pathogen decay during the Managed Aquifer Recharge (MAR) may lead to underestimation of decay of adenovirus and Cryptosporidium and subsequent inaccurate assessment of the required residence time in the aquifer for pathogens to be removed from the recharged water. The decay rate of two indicator bacteria, Salmonella enterica and adenovirus, was found to be significantly slower (t-test, P < 0.05) in the in situ studies when 0.010-μm pore-size membranes were used. The assessment of potential clogging of the diffusion cell membranes showed no clogging of the membranes within the 50-day duration of the study as very little difference in the diffusion rates between new and old membranes was observed.
Conclusions: Laboratory microcosms-based inactivation studies can significantly underestimate the survival potential of enteric viruses in the groundwater. Consequently, in situ studies should be carried out for accurate assessment of potential enteric virus survival in aquifers.
Significance and Impact of the Study: Reliable assessment of potential public health risks from the presence of pathogens in groundwater is essential for proper management of the MAR schemes. The results of this study suggest that in situ assessment of pathogen survival potential in diffusion chambers provided more reliable data for pathogen risk assessment and subsequent risk mitigation plans for MAR schemes.
Keyword Adenovirus
Cryptosporidium parvum
managed aquifer recharge
pathogen inactivation
Salmonella enterica
Enteric Viruses
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Public Health Publications
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