One-step homogeneous magnetic nanoparticle immunoassay for biomarker detection directly in blood plasma

Ranzoni, Andrea, Sabatte, Gwenola, van IJzendoorn, Leo J. and Prins, Menno W. J. (2012) One-step homogeneous magnetic nanoparticle immunoassay for biomarker detection directly in blood plasma. ACS Nano, 6 4: 3134-3141. doi:10.1021/nn204913f


Author Ranzoni, Andrea
Sabatte, Gwenola
van IJzendoorn, Leo J.
Prins, Menno W. J.
Title One-step homogeneous magnetic nanoparticle immunoassay for biomarker detection directly in blood plasma
Journal name ACS Nano   Check publisher's open access policy
ISSN 1936-0851
1936-086X
Publication date 2012-04-24
Sub-type Article (original research)
DOI 10.1021/nn204913f
Open Access Status Not Open Access
Volume 6
Issue 4
Start page 3134
End page 3141
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract Assay technologies capable of detecting low biomarker concentrations in complex biological samples are fundamental for biological research and for applications in medical diagnostics. In this paper we address the challenge to perform protein biomarker detection homogeneously in one single step, applying a minute amount of reagent directly into whole human blood plasma, avoiding any sample dilution, separation, amplification, or fluid manipulation steps. We describe a one-step homogeneous assay technology based on antibody-coated magnetic nanoparticles that are spiked in very small amount directly into blood plasma. Pulsed magnetic fields and a double-linker molecular architecture are used to generate high biomarker-induced binding and low nonspecific binding between the nanoparticles. We demonstrate dose–response curves for prostate specific antigen (PSA) measured in undiluted human blood plasma with a detection limit of 400–500 femtomol/L, in a total assay time of 14 min and an optically probed volume of only 1 nL. We explain the dose–response curves with a model based on discrete binding of biomarker molecules onto the nanoparticles, which allows us to extract reaction parameters for the binding of biomarker molecules onto the nanoparticles and for the biomarker-induced binding between nanoparticles. The demonstrated analytical performance and understanding of the nanoparticle assay technology render it of interest for a wide range of applications in quantitative biology and medical diagnostics.
Keyword Magnetic nanoparticles
One-step homogeneous immunoassay
Whole human blood plasma
PSA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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