A human snoRNA with microRNA-like functions

Ender, Christine, Krek, Azra, Friedlaender, Marc R., Beitzinger, Michaela, Weinmann, Lasse, Chen, Wei, Pfeffer, Sebastien, Rajewsky, Nikolaus and Meister, Gunter (2008) A human snoRNA with microRNA-like functions. Molecular Cell, 32 4: 519-528. doi:10.1016/j.molcel.2008.10.017


Author Ender, Christine
Krek, Azra
Friedlaender, Marc R.
Beitzinger, Michaela
Weinmann, Lasse
Chen, Wei
Pfeffer, Sebastien
Rajewsky, Nikolaus
Meister, Gunter
Title A human snoRNA with microRNA-like functions
Journal name Molecular Cell   Check publisher's open access policy
ISSN 1097-2765
1097-4164
Publication date 2008-11-21
Sub-type Article (original research)
DOI 10.1016/j.molcel.2008.10.017
Volume 32
Issue 4
Start page 519
End page 528
Total pages 10
Place of publication Cambridge, MA, United States
Publisher Cell Press
Language eng
Abstract Small noncoding RNAs function in concert with Argonaute (Ago) proteins to regulate gene expression at the level of transcription, mRNA stability, or translation. Ago proteins bind small RNAs and form the core of silencing complexes. Here, we report the analysis of small RNAs associated with human Ago1 and Ago2 revealed by immunoprecipitation and deep sequencing. Among the reads, we find small RNAs originating from the small nucleolar RNA (snoRNA) ACA45. Moreover, processing of ACA45 requires Dicer activity but is independent of Drosha/DGCR8. Using bioinformatic prediction algorithms and luciferase reporter assays, we uncover the mediator subunit CDC2L6 as one potential mRNA target of ACA45 small RNAs, suggesting a role for ACA45-processing products in posttranscriptional gene silencing. We further identify a number of human snoRNAs with microRNA (miRNA)-like processing signatures. We have, therefore, identified a class of small RNAs in human cells that originate from snoRNAs and can function like miRNAs.
Keyword RNA
PROTEIN
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 05 Nov 2012, 19:06:37 EST by Susan Allen on behalf of Institute for Molecular Bioscience