Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes

Valen, Eivind, Preker, Pascal, Andersen, Peter Refsing, Zhao, Xiaobei, Chen, Yun, Ender, Christine, Dueck, Anne, Meister, Gunter, Sandelin, Albin and Jensen, Torben Heick (2011) Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes. Nature Structural and Molecular Biology, 18 9: 1075-1082. doi:10.1038/nsmb.2091


Author Valen, Eivind
Preker, Pascal
Andersen, Peter Refsing
Zhao, Xiaobei
Chen, Yun
Ender, Christine
Dueck, Anne
Meister, Gunter
Sandelin, Albin
Jensen, Torben Heick
Title Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes
Journal name Nature Structural and Molecular Biology   Check publisher's open access policy
ISSN 1545-9993
1545-9985
Publication date 2011-09-01
Sub-type Article (original research)
DOI 10.1038/nsmb.2091
Volume 18
Issue 9
Start page 1075
End page 1082
Total pages 8
Place of publication New York, United States
Publisher Nature Publishing Group
Language eng
Abstract Efforts to catalog eukaryotic transcripts have uncovered many small RNAs (sRNAs) derived from gene termini and splice sites. Their biogenesis pathways are largely unknown, but a mechanism based on backtracking of RNA polymerase II (RNAPII) has been suggested. By sequencing transcripts 12–100 nucleotides in length from cells depleted of major RNA degradation enzymes and RNAs associated with Argonaute (AGO1/2) effector proteins, we provide mechanistic models for sRNA production. We suggest that neither splice site–associated (SSa) nor transcription start site–associated (TSSa) RNAs arise from RNAPII backtracking. Instead, SSa RNAs are largely degradation products of splicing intermediates, whereas TSSa RNAs probably derive from nascent RNAs protected by stalled RNAPII against nucleolysis. We also reveal new AGO1/2-associated RNAs derived from 3′ ends of introns and from mRNA 3′ UTRs that appear to draw from noncanonical microRNA biogenesis pathways.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 05 Nov 2012, 18:56:49 EST by Susan Allen on behalf of Institute for Molecular Bioscience