High-throughput amplicon sequencing reveals distinct communities within a corroding concrete sewer system

Cayford, Barry I., Dennis, Paul G., Keller, Jurg, Tyson, Gene W. and Bond, Philip L. (2012) High-throughput amplicon sequencing reveals distinct communities within a corroding concrete sewer system. Applied and Environmental Microbiology, 78 19: 7160-7162. doi:10.1128/AEM.01582-12

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Author Cayford, Barry I.
Dennis, Paul G.
Keller, Jurg
Tyson, Gene W.
Bond, Philip L.
Title High-throughput amplicon sequencing reveals distinct communities within a corroding concrete sewer system
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2012-10-01
Year available 2012
Sub-type Article (original research)
DOI 10.1128/AEM.01582-12
Open Access Status File (Publisher version)
Volume 78
Issue 19
Start page 7160
End page 7162
Total pages 3
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract This study investigated the variation in microbially induced concrete corrosion communities at different circumferential locations of a real sewer pipe and the effects of a wastewater flooding event on the community. Three distinct microbial community groups were found in different corrosion samples. The physico-chemical properties of the corrosion layers and the microbial communities were distinct for the cross-sectional positions within the pipe, ie ceiling, wall and tidal zones. The microbial communities detected from the same positions in the pipe were consistent over the length of the pipe, as well as being consistent between the replicate pipes. The dominating ceiling communities were members of the bacterial orders Rhodospirillales, Acidithiobacillales, Actinomycetales, Xanthomonadales and Acidobacteriales. The wall communities were composed of members of the Xanthomonadales, Hydrogenophilales, Chromatiales and Sphingobacteriales. The tidal zones were dominated by eight bacterial and one archaeal order, with the common physiological trait of anaerobic metabolism. Sewage flooding within the sewer system did not change the tidal and wall communities, although the corrosion communities in ceiling samples were notably different, becoming more similar to the wall and tidal samples. This suggests that sewage flooding has a significant impact on the corrosion community in sewers.
Formatted abstract
Microbially induced concrete corrosion (MICC) is an important problem in sewers. Here, small-subunit (SSU) rRNA gene amplicon pyrosequencing was used to characterize MICC communities. Microbial community composition differed between wall- and ceiling-associated MICC layers. Acidithiobacillus spp. were present at low abundances, and the communities were dominated by other sulfur-oxidizing-associated lineages.
Keyword Sewer
concrete
corrosion
flooding
hydrogen sulfide
microbial community
wastewater
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID LP0882016
Institutional Status UQ
Additional Notes Supplemental material (read only) - http://aem.asm.org/content/78/19/7160/suppl/DCSupplemental

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
Advanced Water Management Centre Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 29 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 26 Oct 2012, 00:04:05 EST by Barry Cayford on behalf of Advanced Water Management Centre