Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, beta-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma

Bongiovanni, Laura, Mazzocchetti, Francesca, Malatesta, Daniela, Romanucci, Mariarita, Ciccarelli, Andrea, Buracco, Paolo, De Maria, Raffaella, Palmieri, Chiara, Martano, Marina, Morello, Emauela, Maniscalco, Lorella and Della Salda, Leonardo (2012) Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, beta-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma. BMC veterinary research, . doi:10.1186/1746-6148-8-78

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Author Bongiovanni, Laura
Mazzocchetti, Francesca
Malatesta, Daniela
Romanucci, Mariarita
Ciccarelli, Andrea
Buracco, Paolo
De Maria, Raffaella
Palmieri, Chiara
Martano, Marina
Morello, Emauela
Maniscalco, Lorella
Della Salda, Leonardo
Title Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, beta-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma
Journal name BMC veterinary research   Check publisher's open access policy
ISSN 1746-6148
Publication date 2012-01-01
Sub-type Article (original research)
DOI 10.1186/1746-6148-8-78
Open Access Status DOI
Total pages 24
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2013
Language eng
Formatted abstract
Background: Osteosarcoma (OSA) represents the most common canine primary bone tumour. Despite several pathways have been investigated so far, few molecules have been identified as prognostic tools or potential therapeutic targets, and there is still the need to find out molecular pathways with specific influence over OSA progression to facilitate earlier prognosis and treatment.Aims of the present study were to evaluate the immunohistochemical pattern and levels of expression of a panel of molecules (survivin, beta-catenin, caspase 3 -inactive and active formsand p53) involved in cell cycle and apoptosis regulation in canine OSA samples, known to be of interest in the study also of human OSA, and to detect specific relations among them and with histological tumour grade, disease free interval (DFI) and overall survival (OS).

Results: Nuclear beta-catenin immunostaining was detected in normal osteoblasts adjacent to the tumour, and in 47% of the cases. Cytoplasmic and/or membranous immunostaining were also observed. Nuclear survivin and p53 positive cells were found in all cases. Moderate/high cytoplasmic beta-catenin expression ([greater than or equal to]10% positive cells) was significantly associated with the development of metastasis (P = 0.014); moderate/high nuclear p53 expression ([greater than or equal to]10% positive cells) was significantly associated with moderate/ high histological grade (P = 0.017) and shorter OS (P = 0.049). Moderate/high nuclear survivin expression ([greater than or equal to]15% positive cells) showed a tendency toward a longer OS (P = 0,088).

Conclusions: The present results confirmed p53 as negative prognostic marker, while suggested survivin as a potential positive prognostic indicator, rather than indicative of a poor prognosis. The detection of nuclear beta-catenin immunostaining in normal osteoblasts and the absent/low expression in most of the OSAs, suggested that this pathway could not play a major role in oncogenic transformation of canine osteoblasts. Further studies are needed to confirm these hypotheses.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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Created: Sun, 21 Oct 2012, 22:20:48 EST by Chiara Palmieri on behalf of School of Veterinary Science