Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases

Wheeler, Megan, Chardon, Antoine, Goubet, Astrid, Morihiro, Kunihiko, Tsan, Sze Yee, Edwards, Stacey L., Kodama, Tetsuya, Obika, Satoshi and Veedu, Rakesh N. (2012) Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases. Chemical Communications, 48 89: 11020-11022. doi:10.1039/C2CC36464F


Author Wheeler, Megan
Chardon, Antoine
Goubet, Astrid
Morihiro, Kunihiko
Tsan, Sze Yee
Edwards, Stacey L.
Kodama, Tetsuya
Obika, Satoshi
Veedu, Rakesh N.
Title Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases
Journal name Chemical Communications   Check publisher's open access policy
ISSN 1359-7345
1364-548X
Publication date 2012-10-05
Year available 2012
Sub-type Article (original research)
DOI 10.1039/C2CC36464F
Open Access Status
Volume 48
Issue 89
Start page 11020
End page 11022
Total pages 3
Place of publication Cambridge, United Kingdom
Publisher RSC
Language eng
Abstract Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.
Keyword SeLNA
KOD XL DNA
Primer extension reactions
Polymerase chain reaction (PCR)
Phusion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published on the web: 27 September 2012. Advanced publication.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 12 Oct 2012, 20:40:36 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences