Multiple reaction monitoring for the accurate quantification of amino acids: using hydroxyproline to estimate collagen content

Colgrave, Michelle L., Allingham, Peter G., Tyrrell, Kerri and Jones, Alun (2012). Multiple reaction monitoring for the accurate quantification of amino acids: using hydroxyproline to estimate collagen content. In Michail A. Alterman and Peter Hunziker (Ed.), Amino acid analysis: methods and protocols (pp. 291-303) New York, United States: Humana Press. doi:10.1007/978-1-61779-445-2_23


Author Colgrave, Michelle L.
Allingham, Peter G.
Tyrrell, Kerri
Jones, Alun
Title of chapter Multiple reaction monitoring for the accurate quantification of amino acids: using hydroxyproline to estimate collagen content
Title of book Amino acid analysis: methods and protocols
Place of Publication New York, United States
Publisher Humana Press
Publication Year 2012
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-61779-445-2_23
Series Methods in Molecular Biology
ISBN 9781617794452
9781617794445
ISSN 1064-3745
Editor Michail A. Alterman
Peter Hunziker
Volume number 828
Chapter number 23
Start page 291
End page 303
Total pages 13
Total chapters 28
Collection year 2013
Language eng
Abstract/Summary Multiple reaction monitoring (MRM) mass spectrometry may be regarded as the gold standard methodology for quantitative mass spectrometry and has been adopted for the analysis of small molecules especially within the pharmaceutical industry. It can also be applied to the analysis of peptides and proteins and to the measurement of the basic building blocks of proteins, amino acids. Here, we describe the application of MRM mass spectrometry to the measurement of hydroxyproline after acid hydrolysis of various animal tissues. We show that the measurement of hydroxyproline provides an accurate and reliable estimate of the collagen content of such tissues and may be a useful indicator of meat tenderness.
Keyword Amino acid
Collagen
Hydroxyproline
Mass spectrometry
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 21 Sep 2012, 19:28:04 EST by Ms Imogen Ferrier on behalf of Institute for Molecular Bioscience