Novel pollen allergens of the subtropical Johnson grass (sorghum Halepense) that are important for allergic respiratory disease

Lomas, Victoria, Guru, Preethi, Campbell, Bradley, Loo, Dorothy, Hill, Michelle, Godwin, Ian and Davies, Janet (2012). Novel pollen allergens of the subtropical Johnson grass (sorghum Halepense) that are important for allergic respiratory disease. In: Special Issue: Australasian Society of Clinical Immunology and Allergy (ASCIA) 23rd Annual Scientific Meeting. Australasian Society of Clinical Immunology and Allergy (ASCIA) 23rd Annual Scientific Meeting, Wellington, New Zealand, (7-8). 5–8 September 2012.


Author Lomas, Victoria
Guru, Preethi
Campbell, Bradley
Loo, Dorothy
Hill, Michelle
Godwin, Ian
Davies, Janet
Title of paper Novel pollen allergens of the subtropical Johnson grass (sorghum Halepense) that are important for allergic respiratory disease
Conference name Australasian Society of Clinical Immunology and Allergy (ASCIA) 23rd Annual Scientific Meeting
Conference location Wellington, New Zealand
Conference dates 5–8 September 2012
Proceedings title Special Issue: Australasian Society of Clinical Immunology and Allergy (ASCIA) 23rd Annual Scientific Meeting   Check publisher's open access policy
Journal name Internal Medicine Journal   Check publisher's open access policy
Place of Publication Richmond, VIC, Australia
Publisher Wiley-Blackwell Publishing Asia
Publication Year 2012
Sub-type Published abstract
ISSN 1444-0903
Volume 42
Issue s4
Start page 7
End page 8
Total pages 2
Language eng
Formatted Abstract/Summary
Johnson grass is an important source of grass pollen allergens for patients
with hay fever and allergic asthma in subtropical regions including parts of
Australia, Africa, India, Brazil and southern USA. We aimed to purify the
major allergens of Johnson grass pollen (JGP) and confi rm their clinical
importance.

Allergic sensitivity to JGP in Queensland patients with allergic rhinitis was
examined by skin prick testing. The major allergenic components were purifi
ed from an aqueous extract of JGP by ammonium sulphate precipitation,
hydrophobic interaction and size exclusion chromatography. Sera from 20
non-allergic donors, 25 donors with allergic sensitivities to allergens other
than grass pollen (other allergies) and 64 grass pollen-allergic patients were
tested for IgE reactivity with whole JGP extract and the purified allergens by
ELISA.

Of 48 grass pollen-allergic patients, 77% showed a positive skin prick to JGP.
The predominant allergenic component purifi ed from JGP was the 29 kDa
group 1 allergen, Sor h 1. Serum IgE reactivity between JGP and Sor h 1
was highly correlated (r = 0.9686, p < 0.0001). Serum IgE with Sor h 1 was
observed in 40 out of 41 patients with IgE reactivity to JGP (97.5%) as well
Johnson grass is an important source of grass pollen allergens for patients
with hay fever and allergic asthma in subtropical regions including parts of
Australia, Africa, India, Brazil and southern USA. We aimed to purify the
major allergens of Johnson grass pollen (JGP) and confi rm their clinical
importance.

Allergic sensitivity to JGP in Queensland patients with allergic rhinitis was
examined by skin prick testing. The major allergenic components were purifi
ed from an aqueous extract of JGP by ammonium sulphate precipitation,
hydrophobic interaction and size exclusion chromatography. Sera from 20
non-allergic donors, 25 donors with allergic sensitivities to allergens other
than grass pollen (other allergies) and 64 grass pollen-allergic patients were
tested for IgE reactivity with whole JGP extract and the purifi ed allergens by
ELISA.

Of 48 grass pollen-allergic patients, 77% showed a positive skin prick to JGP.
The predominant allergenic component purifi ed from JGP was the 29 kDa
group 1 allergen, Sor h 1. Serum IgE reactivity between JGP and Sor h 1
was highly correlated (r = 0.9686, p < 0.0001). Serum IgE with Sor h 1 was
observed in 40 out of 41 patients with IgE reactivity to JGP (97.5%) as well
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