Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA

Hochstatter, Julia, Hölzel, Michael, Rohrmoser, Michaela, Schermelleh, Lothar, Leonhardt, Heinrich, Keough, Rebecca, Gonda, Thomas J., Imhof, Axel, Eick, Dirk, Längst, Gernot and Németh, Attila (2012) Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA. Journal of Biological Chemistry, 287 29: 24365-24377. doi:10.1074/jbc.M111.303719

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Author Hochstatter, Julia
Hölzel, Michael
Rohrmoser, Michaela
Schermelleh, Lothar
Leonhardt, Heinrich
Keough, Rebecca
Gonda, Thomas J.
Imhof, Axel
Eick, Dirk
Längst, Gernot
Németh, Attila
Title Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 1083-351X
Publication date 2012-07-13
Sub-type Article (original research)
DOI 10.1074/jbc.M111.303719
Open Access Status File (Publisher version)
Volume 287
Issue 29
Start page 24365
End page 24377
Total pages 13
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Abstract Ribosomal RNA gene transcription, co-transcriptional processing, and ribosome biogenesis are highly coordinated processes that are tightly regulated during cell growth. In this study we discovered that Mybbp1a is associated with both the RNA polymerase I complex and the ribosome biogenesis machinery. Using a reporter assay that uncouples transcription and RNA processing, we show that Mybbp1a represses rRNA gene transcription. In addition, overexpression of the protein reduces RNA polymerase I loading on endogenous rRNA genes as revealed by chromatin immunoprecipitation experiments. Accordingly, depletion of Mybbp1a results in an accumulation of the rRNA precursor in vivo but surprisingly also causes growth arrest of the cells. This effect can be explained by the observation that the modulation of Mybbp1a protein levels results in defects in pre-rRNA processing within the cell. Therefore, the protein may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes.
Keyword Care regulation
Ribosomal RNA (rRNA)
Ribosomal RNA processing
Transcription regulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Pharmacy Publications
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 27 Aug 2012, 21:13:46 EST by Myrtle Sahabandu on behalf of School of Pharmacy