Transcription factors ER71/ETV2 and SOX9 participate in a positive feedback loop in fetal and adult mouse testis

DiTacchio, Luciano, Bowles, Josephine, Shin, Sook, Lim, Dae-Sik, Koopman, Peter and Janknecht, Ralf (2012) Transcription factors ER71/ETV2 and SOX9 participate in a positive feedback loop in fetal and adult mouse testis. Journal of Biological Chemistry, 287 28: 23657-23666. doi:10.1074/jbc.M111.320101

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Author DiTacchio, Luciano
Bowles, Josephine
Shin, Sook
Lim, Dae-Sik
Koopman, Peter
Janknecht, Ralf
Title Transcription factors ER71/ETV2 and SOX9 participate in a positive feedback loop in fetal and adult mouse testis
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 2012-07-01
Sub-type Article (original research)
DOI 10.1074/jbc.M111.320101
Open Access Status File (Publisher version)
Volume 287
Issue 28
Start page 23657
End page 23666
Total pages 10
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
ER71, also known as ETV2, is an ETS transcription factor that is expressed during embryogenesis and in adult testes. We show that Er71 transcription can be up-regulated by SRY, the key determinant of male differentiation. Accordingly, SRY bound to and activated the Er71 promoter, and mutation of a putative SRY binding site abolished this promoter activation. In turn, ER71 was able to bind to the promoter of Sox9, the primary target of SRY and a critical transcription factor for maintenance of the Sertoli cell phenotype. Mutation of the ER71 binding site in the Sox9 promoter suppressed ER71-dependent up-regulation of Sox9 transcription, and a dominant-negative ER71 molecule severely reduced Sox9 transcription in a Sertoli cell line. Conversely, SOX9 bound the Er71 promoter in vivo and Sox9 down-regulation reduced Er71 transcript levels. Together, these data suggest a mechanism by which SRY induces Sox9 and Er71 transcription early in testis differentiation, whereas ER71 and SOX9 participate in an autoregulatory loop to sustain each other's expression after Sry expression has subsided in mice. Thereby, ER71 and SOX9 may affect late testis development as well as the function of the adult male gonad.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 22 Aug 2012, 01:24:33 EST by Susan Allen on behalf of Institute for Molecular Bioscience