Increased blood oxygen level-dependent (BOLD) sensitivity in the mouse somatosensory cortex during electrical forepaw stimulation using a cryogenic radiofrequency probe

Baltes, Christof, Bosshard, Simone, Mueggler, Thomas, Ratering, David and Rudin, Markus (2011) Increased blood oxygen level-dependent (BOLD) sensitivity in the mouse somatosensory cortex during electrical forepaw stimulation using a cryogenic radiofrequency probe. NMR in Biomedicine, 24 4: 439-446. doi:10.1002/nbm.1613

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Author Baltes, Christof
Bosshard, Simone
Mueggler, Thomas
Ratering, David
Rudin, Markus
Title Increased blood oxygen level-dependent (BOLD) sensitivity in the mouse somatosensory cortex during electrical forepaw stimulation using a cryogenic radiofrequency probe
Journal name NMR in Biomedicine   Check publisher's open access policy
ISSN 0952-3480
1099-1492
Publication date 2011-05-01
Sub-type Article (original research)
DOI 10.1002/nbm.1613
Open Access Status File (Author Post-print)
Volume 24
Issue 4
Start page 439
End page 446
Total pages 8
Place of publication West Sussex, United Kingdom
Publisher John Wiley and Sons
Language eng
Formatted abstract
Functional MRI (fMRI) based on the blood oxygen level-dependent (BOLD) contrast is widely used in preclinical neuroscience. The small dimensions of rodent brain place high demands on spatial resolution, and hence on the sensitivity of the fMRI experiment. This work investigates the performance of a 400-MHz cryogenic quadrature transceive radiofrequency probe (CryoProbe) with respect to the enhancement of the BOLD sensitivity. For this purpose, BOLD fMRI experiments were performed in mice during electrical forepaw stimulation using the CryoProbe and a conventional room temperature surface coil of comparable dimensions. Image signal-to-noise ratio (SNR) and temporal SNR were evaluated as quality measures for individual images and for fMRI time series of images, resulting in gains (mean ± standard deviation) with factors of 3.1 ± 0.7 and 1.8 ± 1.0 when comparing the CryoProbe and room temperature coil. The CryoProbe thermal shield temperature did not affect the noise characteristics, with temporal noise levels being 63 ± 16% of the corresponding room temperature value. However, a significant effect on BOLD amplitudes was found, which was attributed to temperature-dependent baseline cerebral blood volumes. Defined local thermal conditions were found to be a critical parameter for achieving an optimal and reproducible fMRI signal. In summary, the CryoProbe represents an attractive alternative for the enhancement of image SNR, temporal SNR and BOLD sensitivity in mouse fMRI experiments.
Keyword Brain Activation
Cryogenic
Mouse
Physiological Noise
Small animal
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 30 NOV 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Centre for Advanced Imaging Publications
 
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Created: Mon, 13 Aug 2012, 21:30:21 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging