Formation of multilayered biopolymer microcapsules and microparticles in a multiphase microfluidic flow

Rondeau, Elisabeth and Cooper-White, Justin J. (2012) Formation of multilayered biopolymer microcapsules and microparticles in a multiphase microfluidic flow. Biomicrofluidics, 6 2: 024125.1-024125.16. doi:10.1063/1.4722296


Author Rondeau, Elisabeth
Cooper-White, Justin J.
Title Formation of multilayered biopolymer microcapsules and microparticles in a multiphase microfluidic flow
Journal name Biomicrofluidics   Check publisher's open access policy
ISSN 1932-1058
Publication date 2012-06-01
Sub-type Article (original research)
DOI 10.1063/1.4722296
Volume 6
Issue 2
Start page 024125.1
End page 024125.16
Total pages 16
Place of publication College Park, MD, United States
Publisher American Institute of Physics
Collection year 2013
Language eng
Formatted abstract
This paper reports the development of a scalable continuous microfluidic-based method for the preparation of multilayered biopolymer microcapsules and microparticles, with a size range of 1 to 100 μm, in a single-layered polydimethylsiloxane-based device. This new approach has been utilised to produce polyethylene oxide (PEO)-based microparticles, layered with subsequent stage wise coatings of polylactide-based block copolymers and polyvinylpyrrolidone. The production process was shown to allow for on-chip encapsulation of protein and vitamin molecules in the biopolymer micro particles, without any further handling after collection from the device. We have studied the release profiles in the case of model molecules of distinctive molecular weights, namely, vitronectin, horse radish peroxidase, and vitamin B 12. We compared the release properties of the microparticles to those from macro-gels of the same materials prepared off-chip. The results indicated that the microparticles have definitively different molecular weight cut-off characteristics, likely due to a denser microstructure within the microparticles compared to the bulk hydrogels. This difference suggests that significant benefits may exist in the use of this method to produce layered biopolymer microparticles in achieving improved controlled release and encapsulation.
Keyword Encapsulation
Enzymes
Hydrogels
Microfluidics
Molecular biophysics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number 024125 Published online: 24 May 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Engineering Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
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