Distribution of 25-hydroxyvitamin D3 in dried blood spots and implications for its quantitation by tandem mass spectrometry

Kvaskoff, David, Ko, Pauline, Simila Henry and Eyles, Darryl (2012) Distribution of 25-hydroxyvitamin D3 in dried blood spots and implications for its quantitation by tandem mass spectrometry. Journal of Chromatography B, 901 47-52. doi:10.1016/j.jchromb.2012.05.040


Author Kvaskoff, David
Ko, Pauline
Simila Henry
Eyles, Darryl
Title Distribution of 25-hydroxyvitamin D3 in dried blood spots and implications for its quantitation by tandem mass spectrometry
Journal name Journal of Chromatography B   Check publisher's open access policy
ISSN 1570-0232
1873-376X
Publication date 2012-06-07
Sub-type Article (original research)
DOI 10.1016/j.jchromb.2012.05.040
Volume 901
Start page 47
End page 52
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Dried blood spots (DBS) are a convenient collection and archiving method for blood specimens. The interest in screening certain analytes in neonatal DBS continues to increase for a variety of paediatric disorders. 25-Hydroxyvitamin D 3 (25OHD 3) is one such analyte. We investigated potential factors that may affect the analysis of 25OHD 3 in prospective cohorts of DBS, such as blood spot volume, hole punch position, and paper type. All of these factors were shown to affect 25OHD 3 levels measured. When blood volumes of <50 μL were spotted, 25OHD 3 concentrations extracted were significantly lower (P < 0.0001). We also observed a chromatographic effect across the surface of blood spots, with 25OHD 3 concentrations significantly higher in outer punched spots compared to those punched from the centre (P < 0.0001). This also correlates with a heavier net weight of blood from outer punched spots (P < 0.0001). This effect was reproducible on two types of paper cards (Whatman 903 ® and FTA ®), and paper type was shown to be highly relevant. We also show that the distribution of 25OHD 3 in whole blood is essentially extracellular, with over 98% of 25OHD 3 residing in the serum component. This may potentially explain why the diffusion properties of blood and type of chromatographic paper may significantly influence the distribution of such analytes in DBS. These factors should be taken into consideration for the prospective collection of DBS and analysis of 25OHD 3 in DBS.
Keyword 25-Hydroxyvitamin D
Dried blood spots
Fta
Lc-ms/ms
Punch location
Whatman 903
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 7 June 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2013 Collection
 
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Created: Wed, 11 Jul 2012, 00:09:05 EST by Debra McMurtrie on behalf of Queensland Brain Institute