Molecular mapping of qualitative and quantitative loci for resistance to Leptosphaeria maculans causing blackleg disease in canola (Brassica napus L.)

Raman, Rosy, Taylor, Belinda, Marcroft, Steve, Stiller, Jiri, Eckermann, Paul, Coombes, Neil, Rehman, Neil, Lindbeck, Kurt, Luckett, David, Wratten, Neil, Batley, Jacqueline, Edwards, David, Wang, Xiaowu and Raman, Harsh (2012) Molecular mapping of qualitative and quantitative loci for resistance to Leptosphaeria maculans causing blackleg disease in canola (Brassica napus L.). Theoretical and Applied Genetics, 125 2: 405-418. doi:10.1007/s00122-012-1842-6


Author Raman, Rosy
Taylor, Belinda
Marcroft, Steve
Stiller, Jiri
Eckermann, Paul
Coombes, Neil
Rehman, Neil
Lindbeck, Kurt
Luckett, David
Wratten, Neil
Batley, Jacqueline
Edwards, David
Wang, Xiaowu
Raman, Harsh
Title Molecular mapping of qualitative and quantitative loci for resistance to Leptosphaeria maculans causing blackleg disease in canola (Brassica napus L.)
Formatted title
Molecular mapping of qualitative and quantitative loci for resistance to Leptosphaeria maculans causing blackleg disease in canola (Brassica napus L.)
Journal name Theoretical and Applied Genetics   Check publisher's open access policy
ISSN 0040-5752
1432-2242
Publication date 2012-07-01
Sub-type Article (original research)
DOI 10.1007/s00122-012-1842-6
Volume 125
Issue 2
Start page 405
End page 418
Total pages 14
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Blackleg, caused by Leptosphaeria maculans, is one of the most important diseases of oilseed and vegetable crucifiers worldwide. The present study describes (1) the construction of a genetic linkage map, comprising 255 markers, based upon simple sequence repeats (SSR), sequence-related amplified polymorphism, sequence tagged sites, and EST-SSRs and (2) the localization of qualitative (race-specific) and quantitative (race non-specific) trait loci controlling blackleg resistance in a doubled-haploid population derived from the Australian canola (Brassica napus L.) cultivars Skipton and Ag-Spectrum using the whole-genome average interval mapping approach. Marker regression analyses revealed that at least 14 genomic regions with LOD ≥ 2.0 were associated with qualitative and quantitative blackleg resistance, explaining 4.6-88.9 % of genotypic variation. A major qualitative locus, designated RlmSkipton (Rlm4), was mapped on chromosome A7, within 0.8 cM of the SSR marker Xbrms075. Alignment of the molecular markers underlying this QTL region with the genome sequence data of B. rapa L. suggests that RlmSkipton is located approximately 80 kb from the Xbrms075 locus. Molecular marker-RlmSkipton linkage was further validated in an F 2 population from Skipton/Ag-Spectrum. Our results show that SSR markers linked to consistent genomic regions are suitable for enrichment of favourable alleles for blackleg resistance in canola breeding programs.
Keyword Blackleg
Crucifiers
Resistance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
School of Agriculture and Food Sciences
Official 2013 Collection
 
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